高通量指的是利用电脑和相关的机器将那些大量的序列进行分析,一次可以进行多个样本的检测。高通量测序技术(High-throughput sequencing)又称“下一代”测序技术("Next-generation" sequencing technology),或大规模平行测序(Massively parallel sequencing,MPS)。区别于传统Sanger(双脱氧法)测序,能够一...
高通量测序(High-Throughput Sequencing, HTS),又称下一代测序(Next-Generation Sequencing, NGS),是一种先进的DNA测序技术,能够在短时间内同时对大量DNA分子进行序列测定。相比于传统的Sanger测序,HTS具有速度快、通量高、成本低等优点,广泛应用于基因组学、转录组学、表观遗传学等领域。以下是高通量测序的几个...
Years of research have demonstrated that high-throughput sequencing technology has the potential to dramatically accelerate biological research, by enabling the comprehensive analysis of genomes and transcriptomes. This paper aims to introduce the high-throughput sequencing methodology, particularly focusing on...
lastColumn <- file1[, ncol(file1)] #column1 <- file1[, 1]:将file1数据框的第一列提取出来,并将其赋值给column1变量。 #lastColumn <- file1[, ncol(file1)]:将file1数据框的最后一列提取出来,并将其赋值给lastColumn变量 mergedData <- data.frame(column1, lastColumn) files <- c(file2,...
high throughput sequencing名词解释high throughput sequencing名词解释 高通量测序(high throughput sequencing),也称为大规模并行测序技术,是一种快速且有效的DNA或RNA测序方法,能够在较短时间内对大量的DNA或RNA分子进行测序分析。其主要特点是能够同时并行测序多个DNA或RNA样本,同时可以快速地生成大规模的测序数据。
High throughput sequencing DNA databases are available for severaldecapodspecies to underlie genomic andtranscriptomicresponses to environmental, pathological and physiological stressors (Table 1). Although at the onset of this review, studies on the investigation of digestive system are scarce (Wang...
Fungi are major ecological players in both terrestrial and aquatic environments by cycling organic matter and channelling nutrients across trophic levels. High-throughput sequencing (HTS) studies of fungal communities are redrawing the map of the fungal
The results revealed the vsiRNAs profile of an ASGV variant, as well as the alteration of the tRNA-derived sRNA profile in response to latent virus infection. It also highlights the importance of library preparation in the interpretation of high-throughput sequencing data....
High-throughput sequencing technologies are now routinely being applied to a wide range of important topics in biology and medicine, often allowing researchers to address important biological questions that were not possible before. In this review, we discuss these innovative new approaches-including ...
(CGr) leaves using high-throughput sequencing method. The potential miRNA target genes were predicted and annotated using the NR, Swiss-Prot, GO, COG, KEGG, KOG and Pfam databases. The main biological processes regulated by miRNAs were revealed. The miRNAs which potentially play important roles ...