Common steps (e.g., deprotection and washing) are performed batchwise on the synthesis crowns, in order to optimize economy, and throughput. Even after trifluoroacetic acid (TFA) cleavage, samples continue to be handled in parallel during ether precipitation, washes, re-dissolving, and ...
Bottom-up synthesis of prototissues helps us to understand the internal cellular communications in the natural tissues and their functions, as well as to improve or repair the damaged tissues. The existed prototissues are rarely used to improve the funct
Here, we develop a general strategy for ultra-high-throughput mapping of the chemical space of asymmetric catalysis by escaping the time-consuming chiral chromatography separation. The ultrafast ( ~ 1000 reactions/day) and accurate (median error < ±1%) analysis of enantiomeric excess are...
High-throughput construction method for expression vector of peptides for NMR study suited for isotopic labeling. Tenno T; GOda N;Tateishi Y;.High-throughput construction method for expression vector of peptides for NMR study suited for isotopic labeling.Protein Eng ... T Tenno,N Goda,Y ...
Peptidoglycan composition and density were maintained after we systematically altered cell size in Escherichia coli using the antibiotic A22, indicating that cell shape is largely decoupled from the biochemistry of peptidoglycan synthesis. High-throughput, sensitive UPLC combined with our automated software ...
Because the number of printable additives is immense, one can cover a large combinatorial space through combinatorial high-throughput screening over a multitude of hydrogel prepolymerization mixtures (including, for example, peptides that promote cell adhesion, fibronectin, laminin, hyaluronic acid, dextra...
While there are many potential avenues that remain to be explored (see Outstanding questions), the palette of selection techniques now available to researchers, combined with the advent of low-cost NGS to allow high-throughput identification and analysis of the variants unmasked by these screens, ...
Self-encoding Functional Resin Applying for Combinatorial Chemistry and High Throughput Screening A novel solid phase organic synthesis resin was synthesized for combinatorial high-throughput screening, which based on FTIR spectra self-encoding function... DU Lei,TS Chen - 《武汉理工大学学报(材料科学版...
A high-throughput peptidomic strategy to decipher the molecular diversity of cyclic cysteine-rich peptides Aida Serra*, Xinya Hemu*, Giang K. T. Nguyen, Ngan T. K. Nguyen, Siu Kwan Sze & James P. Tam Cyclotides are plant cyclic cysteine-rich peptides (CRPs)...
We developed a novel strategy for generating phosphorylation site-specific monoclonal antibodies by cloning immunoglobulin genes from single plasma cells that were fixed, intracellularly stained with fluorescently labeled peptides and sorted without causing RNA degradation. Our high-throughput fluorescence ...