DNA data storage consists of the following steps: encoding, DNA synthesis (i.e., writing), preservation, retrieval, DNA sequencing (i.e., reading), and decoding. Out of these steps, DNA synthesis presents a bottleneck due to imperfect coupling efficiency, low throughput, and excessive use of...
高通量测序(High-Throughput Sequencing, HTS)，又称下一代测序(Next-Generation Sequencing, NGS)，是一种先进的DNA测序技术，能够在短时间内同时对大量DNA分子进行序列测定。相比于传统的Sanger测序，HTS具有速度快、通量高、成本低等优点，广泛应用于基因组学、转录组学、表观遗传学等领域。以下是高通量测序的几个...
This review discusses the current methods of chemical DNA synthesis and gene assembly, as well as the latest engineering tools, technologies and trends which could potentially lead to breakthroughs in the development of accurate, low-cost and high-throughput gene synthesis technology. The capability ...
A new porous metal-organic framework (MOF)-type aluminum pyromellitate (MIL-121 or Al(OH)[H(2)btec]·(guest), (guest = H(2)O, H(4)btec = pyromellitic acid) has been isolated by using a high-throughput synthesis method under hydrothermal conditions. Its structure was determined from po...
Bottom-up synthesis of prototissues helps us to understand the internal cellular communications in the natural tissues and their functions, as well as to improve or repair the damaged tissues. The existed prototissues are rarely used to improve the function of living tissues. We demonstrate a meth...
A directed evolution screen must maintain a link between genotype and phenotype as DNA sequencing is utilised to identify positive mutants. Can we develop accurate protein sequencing methods that eliminate the need for this linkage? Can we develop high-throughput long-read sequencing techniques for ass...
DNA replication initiates from replication origins firing throughout S phase. Debate remains about whether origins are a fixed set of loci, or a loose agglomeration of potential sites used stochastically in individual cells, and about how consistent thei
High throughput methods were employed for the physical vapour deposition (PVD) and electrochemical screening of random and equilibrated (annealed at 300 ºC for 15 minutes) binary metal alloys for the hydrogen evolution reaction (HER) and the hydrogen oxidation reaction (HOR). Results are presented...
Chapter eleven - Industrial Scale Gene Synthesis Meanwhile, high-throughput design and synthesis of extremely comprehensive DNA sequences have evolved into an enabling technology already indispensable in various... F Notka,M Liss,R Wagner - Elsevier Science & Technology 被引量: 10发表: 2011年 Devel...
15High Throughput Sequencing High Throughput Sequencing(HTS) technologies are recent and continuously developing techniques that enable the sequencing of multiple DNA molecules in parallel, creating large data sets in a shorter time than ever possible, moreover the costs of HTS are decreasing. The...