HIGH-RESOLUTION HLA TYPINGThe present invention relates to improved methods and kits for typing HLA class I and class II loci using DNA amplification and sequencing.ALIZADEH MehdiSEMANA Gilbert
对于通过 MinION 上的扩增子测序进行的基于一致性的 HLA 分型,我们开发了 Athlon 管道,首先通过两个过程在 I 类 HLA 位点识别一个(纯合)或两个(杂合)候选等位基因:1)读取映射到集合IMGT/HLA 数据库中的已知参考序列,以及 2) 比较抗原和等位基因水平的总读取深度。接下来,使用 Freebayes用于重新排列到每个候选等...
Figure 1. HLA solution from Nanodigmbio. A.HLAtyping Panel v1.0 is based on the reference genome exon probes, supplemented with polymorphic probes according to the HLA database, to achieve effective capture of all types;B.HLAtyping Panel v2.0 can cover all known reference sequences in the l...
Kögler, G., Enczmann, J., Rocha, V.et al.High-resolution HLA typing by sequencing for HLA-A, -B, -C, -DR, -DQ in 122 unrelated cord blood/patient pair transplants hardly improves long-term clinical outcome.Bone Marrow Transplant36, 1033–1041 (2005). https://doi.org/10.1038/sj...
Here we propose a combination of PCR-SSO, PCR-SSP and PCR-RFLP to achieve high resolution typing of the HLA-DR alleles. The low/intermediate resolution typing can be obtained by any method; in our laboratory we use PCR-SSO. As a second step we also perform a group specific amplification...
HLA typing in large scale of cord blood samples with PCR-SSOP; 应用PCR-SSOP技术进行大样本脐血HLA基因分型的探讨 更多例句>> 3) High-resolution DNA typing 高分辨基因分型4) HLA DRB genotyping HLA-DRB基因分型 例句>> 5) HLA-DPB genotyping HLA-DPB_1基因分型6...
求翻译:highresolution molecular HLA typing是什么意思?待解决 悬赏分:1 - 离问题结束还有 highresolution molecular HLA typing问题补充:匿名 2013-05-23 12:21:38 高分辨率的分子HLA分型 匿名 2013-05-23 12:23:18 HLA分子高分辨率 匿名 2013-05-23 12:24:58 高分辨率分子HLA键入 匿名 2013-...
Single step amplification combined to BW4/6 allows high resolution typing of this complex group of alleles. This procedure can be extrapolated to other HLA-B CREGs (e.g. B7, B8, CREGs). It thus constitutes a novel approach for comprehensive B locus typing....
The main aim of this study was to develop and evaluate a rapid and cost-effective method for high-resolution HLA class I typing using Oxford Nanopore sequencing. The study focused on detecting HLA alleles that are associated with drug hypersensitivity, such as HLA-B15:02, HLA-...
Two studies have been performed to examine HLA molecular polymorphism in the Macedonian population, so far. The present study is the first to be performed in Macedonia using high-resolution sequence-based method for direct HLA typing. The study included 158 unrelated healthy volunteers of Macedonian...