mRNA was quantified using the CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, California, USA). qRT-PCR analysis was performed using the following primer sets: (F) 5ʹ-CGACCTGTTAGACGCTGATT-3ʹ and (R) 5ʹ-GTTCAGCGGTAACACGGATT-3ʹ (gapA), (F) 5ʹ-CGCGCTTATCGTGAAG...
and primers for rat PAI-1 (5'-ATGAGATCAGTACTGCGGACGCCATCTTTG-3', 5'-AC- AAGTCTGATGGTAGCACCATCTCCGTGC-3') or β-actin (5'-AATGCCTCCTGCACCACCAAC-3', 5'-AAGGCCATGC- CAGTGAGCTTC-3'). PCR reactions with SYBR Green I and primers were performed in a 20 μl volume with 2 μ...
Lentiviral vectors expressing specific short hairpin RNA (shRNA) targeting METTL3 (5′-GGTTCGTTCCACCAGTCATAA-3′) or METTL14 (5′-GCTGGACTTGGGATGATATTA-3′), along with a puromycin resistance gene, were provided by the Zhejiang Ying Yang Pharmaceutical R&D Center. 293T cells were seeded at...
LABFAC Dimension(L*W*H) 162*135*147cm Automatic Grade Automatic Color & Page Two colors Condition New Machinery Test Report Provided Video outgoing-inspection Provided Warranty of core components 1 Year Core Components Bearing, Motor Max. Power 2000W Connectivity USB, SD card, U disk Max. No...
5 ACGTGAGCTCGGTGTACCTGCTGTTAAAGGAGGT Forward SacI Normal: Δ384 Normal 6 ACGTGAGCTCAATGGGTGAGTAGGGATGGATTACA Forward SacI Normal: Δ543 Normal 7 ACGTGAGCTCAATGGGTGAGTAGGGATGGATTA Forward SacI In/del: Δ148 In/del 8 AATGGGTGAGTAGGGATGGATTACACC Inverse-F In/del: Δin/del-F In/del 9 CC...
cells/ul. For encapsulation, cell and bead solutions were loaded at 30 ul/min and encapsulation oil was loaded at 200 ul/min. Libraries were sequenced on a NextSeq 500 (Illumina) (21x8x0x138) at a 3pM concentration with custom Read1 Drop-seq primers (GCCTGTCCGCGGAAGCAGTGGTATCAACGCAGAGT...
(forward tail CCATCTCATCCCTGCGTGTCTCCGACTCAG-index-GAT; reverse tail CCTCTCTATGGGCAGTCGGTGAT-index) for sequencing using the Ion Torrent system (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA). PCR amplification was performed in a reaction volume of 5 µl containing Hot FIRE...
For rs776746, the context sequence is [VIC/FAM]: ATGTGGTCCAAACAGGGAAGAGATA[T/C]TGAAAGAC AAAAGAGCTCTTTAAAG. Reagents for LC‒MS/MS analysis Atorvastatin was a generous gift from EIPICO, Al Sharkia, Egypt, whereas rosuvastatin (internal standard [IS]) was a lavish gift from Mash ...
(5′-CTCCGGTTTCGATTGAGAAG-3′), reverse primer (5′-AGTTGTTCACGGCTCTTCGT-3′); Egr1, forward primer (5′-TTATCCCAGCCAAACTACCC-3′), reverse primer (5′-CAGAGGAAGACGATGAAGCA-3′); Col1a1, forward primer (5′-GGAAGAGCGGAGAGTACTGG-3′), reverse primer (5′-CATGCTCTCTCCAAACCA...
PRRSV 5′-CTAAGAGAGGTGGCCTGTCG′ and 5′-GAGACTCGGCATACAGCACA-3′; Marc-145-GAPDH 5′-CCTTCCGTGTCCCTACTGCCAAC-3′ and 5′-GACGCCTGCTTCACCACCTTCT-3′; PAM-GAPDH 5′-TCTGGCAAAGTGGACATT-3′ and 5′-GGTGGAATCATACTGGAACA-3′; IFNβ 5′-CACGACAGCTCTTTCCATGA-3′ and 5′-AGCCAG...