The median number of infused CD3+ cells in patients who engrafted within the first 42 days after UCBT was 5.7 × 106/kg (range, 1.8 to 53.9 × 106/kg; IQR, 3.5 to 11.4 × 106/kg), and that for patients without evidence of neutrophil engraftment within the first 42 days after UCBT...
Quantification of lymphocyte sub-populations showed that the increased number of BAL CD3-positive lymphocytes in HIV-PCP patients was mainly due to a significantly increased number of CD8-positive T cells, while the pulmonary CD4-positive T cells were decreased both in relative and absolute numbers....
Meanwhile, evident immunosuppression could be confirmed at 72 h post-CLP operation, as supported by dramatically diminished CD3+ cells as well as CD4+ T lymphocytes, along with substantial expansion of CD3+CD4+Foxp3+ regulatory T cells (Tregs) (Fig. 4d–f). In addition, it was significantly...
However, when Tregs were expressed as a proportion of CD4+ T cells, there were no significant correlations with either TNC, CD34+, CD3+, CD19+ or CD3−CD56+ cell dose. PBSC grafts from sibling donors contained higher numbers of Tregs (4.35 vs 3.48 × 108; P = 0.01) and higher ...
CD3 levels also generally correlated with CD8 infiltration, and CD8 T cells were more abundant than FOXP3+ Tregs (Supplementary Fig. 8). Fig. 4: Pretreatment tumor T cell infiltrates and responses to CAR-T therapy. a, Distribution of tumors with high, intermediate or negative/low CD3 ...
A high-resolution, absolute-dated Holocene and deglacial Asian monsoon record from Dongge Cave, China We present a continuous record of the Asian monsoon over the last 16 ka from δ 18O measurements of stalagmite calcite. Over 900 oxygen isotopic measuremen... CA Dykoski,RL Edwards,C Hai,....
Visceral leishmaniasis (VL) is severe and potentially fatal. Brazil is one of the countries with the greatest endemicity for the disease in the world. The reduction of CD4+ T lymphocytes, B cells activation and high levels of inflammatory cytokines (IL-6
As T cells produce a burst of ROS during cellular activation, we postulated that a comparison of resting (unstimulated) versus activated (anti-CD3 and anti-CD28 stimulated) T cell redoxomes would reveal changes in the oxidation state of cysteines involved in cellular activation. To test this mo...
To restrict the range strictly to within a four-fold perturbation, we imposed a maximum absolute value of 2 on the resulting value: $$z_i = {{{\mathrm{max}}}\left( { - 2,\min \left( {2,y_i} \right)} \right)$$ The perturbation of each original estimate was then computed as ...
Absolute cell numbers were obtained by using Trucount tubes (Becton Dickinson). T-regulatory phenotyping was performed in peripheral blood mononuclear cells purified by Ficoll gradient. The following fluorochrome-labeled monoclonal antibodies were used for cell surface staining: CD3 (clone SK7) and CD25...