HIF1-Alpha激活的基因包括:VEGF-促进血管生成;GLUT1(葡萄糖转运蛋白-1)-激活葡萄糖转运;LDHA(乳酸脱氢酶)-参与糖酵解途径;和Epo-诱导红细胞生成。HIF1-Alpha还可以激活NOS(一氧化氮合成酶)的转录,从而促进血管生成和血管舒张。研究表明,其他的一些蛋白,如ARNT2和MOP3(Pas超家族3的成员
脯氨酰羟化酶的所有三种蛋白都可以在ODD中两个脯氨酸位点之一(Pro-402和Pro-564)上羟化HIF1-Alpha。HIF2-Alpha和HIF3-Alpha中也存在类似的脯氨酰残基。 在有氧的情况下,EGLN蛋白会被激活,并能使HIF1-Alpha的ODD结构域羟基化,从而使pVHL结合并多聚泛素化HIF(参考文献3)。VHL是一种较大复合物的一部分,该...
HIF1-Alpha激活的基因包括:VEGF-促进血管生成;GLUT1(葡萄糖转运蛋白-1)-激活葡萄糖转运;LDHA(乳酸脱氢酶)-参与糖酵解途径;和Epo-诱导红细胞生成。HIF1-Alpha还可以激活NOS(一氧化氮合成酶)的转录,从而促进血管生成和血管舒张。研究表明,其他的一些蛋白,如ARNT2和MOP3(Pas超家族3的成员)也是可以与HIF1-Alpha形...
The α-subunits that form the oxygen-sensitive component of the hypoxia-inducible factor (HIF) transcription factor have unique and overlapping roles in mediating cellular responses to hypoxia. Surprisingly, they can also have opposing roles, and the dif
Structure, expression patterns, and DNA binding of HIF subunits Both HIF-1 and HIF-2 are heterodimeric proteins composed of an O2-sensitive alpha subunit and a constitutively expressed beta subunit. Full-length HIF-1α or HIF-2α consists of 826 or 870 amino acids, respectively. Both HIF-1...
Interestingly, HIFα is observed to have undergone multiple duplication events coinciding with the evolution of vertebrate species7. In humans, there are three HIFα proteins, HIF1α, HIF2α, and HIF3α, among which the former two are best characterized. There are considerable similarities ...
HIF1Alpha_Pathway之经典信号通路.ppt,HSP70 HIF1a Pathway Elongin-B Elongin-C Elongin-B p53 MKK3 MKK6 ERK2 FIH1 Ref1 PHD2 PHD1 TCP1a HSP90 MDM2 Ub HIF1a HIF1a HIF1a HIF1a HIF1a HIF1a Per1 JAB1 Ub Ub Ub Ub Ub VHL VHL ARNT HNF4a2 SRC1 HIF1a Degradation HIF1a
This hypoxic response is mediated by hypoxia-inducible factors (Hif1α, Hif2α, and Hif3α) and their obligate partner, Hif1β (Arnt). We show that mice lacking Hif1β in fat (FH1βKO) are lean, exhibit reduced adipocyte size, and are protected from age- and diet-induced glucose ...
2 HIF 1的调节 在体内HIF 1的活性调节主要有4个方面,即HIF 1mR NA 的表达水平调节;HIF 1蛋白水平的调节;HIF 1二聚化和DNA 结合活性的调节;HIF 1转录活性的调节。铁离子螯合剂、钴、镍等金属及部分抗氧化剂均能使HIF 1表达增加,但低氧是最主要的生理性HIF 1表达调节因子。在正常氧浓度条件...
Targeting of HIF-alpha to the von Hippel–Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation Science (2001) T. Kietzmann et al. Perivenous expression of the mRNA of the three hypoxia-inducible factor alpha-subunits, HIF1alpha, HIF2alpha and HIF3alpha, in rat liver Biochem. J...