The plate was shaken for 3 s at an amplitude of 1 mm before resulting luminescent signals, proportional to caspase activity, was measured using a luminometer (Tecan Infinite®200 PRO, Tecan, Männedorf, Switzerland). 4.8. Statistical Analysis All data of the in vitro experiments were ...
Every dependent variable exhibited a p-value less than 0.00001, unequivocally indicating the models' substantial significance. Additionally, the measured mass transfer fluxes aligned remarkably well with the model's calculated values. Regarding the R2 and Adjusted R2 values, they are 0.9822 and 0.9795,...
HER2.125I hu 7C2, specific activity 34 μCi/μg, was added to cells in the presence of cold hu 7C2, at a concentration of 500 nM, with 1:3 serial dilutions. Cells were incubated at room temperature for 2 hr. Radioactivity was measured using a Packard Gamma Counter (Perkin Elmer...
(A) Mean HER2 RQ for SK-BR-3 (HER2 amplified) and MDA-MB-231 (unamplified) cell line DNA, measured using 3, 5 and 9 independent replicates. (B) Mean HER2 RQ for three HER2 IHC-negative (patients 1–3) and three HER2 3+ (patients 3–6) tumour DNA samples. DNA was diluted 50-...
(HER2, orERBB2) status. HER2 is a major driving molecule and a therapeutic target of breast cancer, and HER2 status is determined by immunohistochemistry (IHC) and in situ hybridization (ISH) methods in the clinic3,4. Although HER2-positive diseases (defined as IHC 3+ or 2+ with ISH+)...
OS was measured from study entry until death from any cause. Surviving patients were censored at the date of last contact. Kaplan-Meier curves and log-rank tests were used for comparing time to event distributions. Cox proportional hazard regression analyses, adjusted for treatment, were performed...
This approach was considered the most suitable given the exploratory nature of the analyses and considering that the measured biomarkers were selected based on biological relevance. In addition, each category of biomarker was measured in different subsets of patients, and consequently comparisons between ...
Tumor responses were evaluated by independent radiologists every 6 weeks using the RECIST criteria for 1 year and every 9 weeks thereafter. The left ventricular ejection fraction was measured using multi-gated acquisition scans or echocardiography every 9 weeks during the first eight cycles and every ...
6A,B). Furthermore, we measured caspase-3 enzymatic activity and found that AC009283.1 knockdown significantly increases the level of caspase-3 activity at day four, compared with negative control cells (shRNA NC) (Fig. 6C). These results suggest that AC009283.1 promotes apoptosis resistance in...
For example, in vitro cytotoxicity of ADCs is typically measured at or after three days of continuous incubation with cells. This allows for sufficient time for killing of cells during cellular growth. The methods to determine intracellular drug levels are designed for intact live cells. Thus the...