The scFv 4D5-dibarnase exerted adoi:10.1007/s10637-009-9329-2TarasG.BalandinEvelinaEdelweissNataliaV.AndronovaElenaM.Springer USInvestigational New DrugsBalandin TG, Edelweiss E, Andronova NV, Treshalina EM, Sapozhnikov AM & Deyev SM (2011) Antitumor activity and toxicity of anti-HER2 immuno...
4D5 scFv was amplified by PCR, based on the HER2 CAR vector purchased from Promab (PM-CAR-1024), linked to CD28 transmembrane and CD28 and CD3ζ intracellular signaling domains, and sub-cloned into pHR-EF1αp lentiviral vector. The tobacco etch virus (TEV) protease [15] was PCR amplified...
7.虽然现有靶向her2的抗体也有串联单链可变片段(scfv),由于不含fc体内半衰期更短,且受限理化条件影响大规模生产。另外,其对her2表达较低的癌症患者疗效不佳,因此,开发肿瘤靶向的her2抗体药物如双特异性抗体仍然很有必要。 技术实现要素: 8.本发明所要解决的技术问题是为了克服现有技术中缺少有效靶向her2的双特异性抗...
Our results demonstrated that Gigantoxin-4-4D5 scFv exerted a highly cytotoxic effect on the HER2/neu-positive ovarian carcinoma SK-OV-3 cell line. And the hemolytic activity was weaker, making it safe for normal cells. The results of immunofluorescence analysis showed that this novel ...
which binds a target protein other than HER230. Greene et al. reported the results of a breast cancer xenograft model, demonstrating the antitumor efficacy of a HER2 scFv and an IFN-γ engineered protein31. This new compound showed increased antitumor activity when compared to the 4D5 HER2-dir...
在一个实施方案中,包含her2结合域的抗her2抗体为抗体片段,例如fv,fab,fab’,scfv,双抗体,或f(ab’)2片段。在另一个实施方案中,该抗体为基本上全长抗体,例如igg1抗体,igg2a抗体或本文中定义的其它抗体类或同种型。本文中提供的是抗her2抗体,其包含her2结合域,该her2结合域包含轻链可变域,该轻链可变域包含与hu...
In our laboratory, we selected by phage display and characterized two human anti-Id scFv fragments, 40 and 69, which mimic the human HER2 antigen and can induce humoral anti-HER2 response in BALB/c mice [96]. More recently, we selected by phage display and characterized 1HE, a llama anti...
DARPins were bound to SKBR-3 cells at 50 nm, and soluble scFv 4D5 was added to a concentration of 1 μm. Binding was detected as in D. The mean fluorescence intensity was plotted. The DARPins termed E3_5 and E3_19 were not selected but had been picked randomly from the naive ...
scFv。附图说明[0031]通过参考阐述了说明性实施例的以下详细描述,将获得对本发明的特征和优点的更好理解,在所述实施例中利用了本发明的原理,并且在附图中:[0032]图1为比较不同嵌合抗原受体与所述靶HER2的活性的表。分别相较于FRP5和4D5,FRP5*和4D5*在VH结构域中各自具有单个氨基酸变化。IgG4(EQ)具有突变CH2...
重组免疫毒素Gigantoxin-4-4D5 scFv的制备,体外活性研究及链接优化 免疫毒素是靶向抗肿瘤药物一个新的研究热点,具有良好的应用前景.传统的免疫毒素主要是通过进入细胞内部而发挥作用,在实体瘤中效果不理想,有一定的局限性.本文利用柔性... 吕欣欣 - 《华东理工大学》 被引量: 0发表: 2016年 ...