对于胃癌,首选的检测方法是IHC,而乳腺癌的检测则没有明确指出应该优先使用IHC还是FISH方法。 Her-2阳性具体到药物上也有差别 以曲妥珠单抗为例,这种药物的作用机制是通过与HER-2蛋白结合来发挥作用,因此在评估治疗效果时,首选的检测方法是IHC,它用于检测HER-2蛋白的过度...
对于胃癌,首选的检测方法是IHC,而乳腺癌的检测则没有明确指出应该优先使用IHC还是FISH方法。 Her-2阳性具体到药物上也有差别 以曲妥珠单抗为例,这种药物的作用机制是通过与HER-2蛋白结合来发挥作用,因此在评估治疗效果时,首选的检测方法是IHC,它用于检测HER-2蛋白的...
Patients were divided into three cohorts based on the degree of HER2 positivity: HER2+ with immunohistochemistry (IHC) scoring 3+ or IHC2+/in-situ-hybridization (ISH)+ (cohort A, n = 53) HER2IHC2+/ISH– (cohort B, n = 7) HER2IHC1+ (cohort C, n = 18) All patients received T-D...
HER2 IHC scoring was carried out according to the updated ASCO/CAP guidelines for breast and gastroesophageal adenocarcinoma by two pathologists independently. Data analysis was performed using SPSS v.24.Results:A total of 104 cases of cervical cancer, 102 cases of endometrial cancer and 103 cases ...
1免疫组化检测法(IHC) 免疫组化是利用各种标记物连结到抗体(抗原) 分子上,以检测和定位组织内相应抗原(抗体)的技 术,免疫组化是目前检测Her一2的主要技术方法,但 其检测结果受到许多技术性因素的影响: 作者简介:夏丹(1978一),女,湖北省武汉市人,2002年毕业于北华 ...
Her-2基因是癌基因, 定位于人染色体17q21上, 编码具有酪氨酸激酶活性的细胞膜糖蛋白, 属于酪氨酸激酶Ⅰ型受体家族, 参与细胞的分裂、生长、繁殖的调控, 与细胞运动、细胞生存能力的增强及癌细胞转移有关. 在正常情况下处于非激活状态, 当Her-2受到某些致癌因素的作用后, 其结构或表达失控而被激活, 转而具有肿瘤...
19 Moreover, IHC scoring system is subjective and this can lead to reproducibility issues.20,21 Therefore, to improve the accuracy and reproducibility of HER family member quantification in tumours, we developed a time-resolved Förster resonance energy transfer (TR-FRET) method that allows the ...
Excellent interlaboratory agreement for HER-2/neu IHC was attained using the same primary antibody to HER-2/neu, even without standardization of assay method or scoring criteria. However, standardization of these parameters remains an important objective to optimize interlaboratory agreement. 展开 关键词...
Therefore, antigen loss may occur in up to 20% of HER-2/neu positive samples. Several other conditions can contribute to false-positive or false-negative IHC results, including tissue processing, reagent variability, antigen retrieval methods, scoring interpretation, tumor heterogeneity, and the semi...
荧光原位杂交与免疫组织化学检测胃癌组织中HER-2基因及蛋白表达一致性