非酒精性脂肪性肝病(NAFLD)是以肝实质细胞脂肪蓄积和脂肪变性为特征的一种代谢紊乱性疾病。现有多数文献中记载了:胰高血糖素样肽-l(GLP-1)受体激动剂(Ra),如降糖药物利拉鲁肽(Lira)既能改善肝脂肪变性及胰岛素抵抗,还可抑制饱食中枢、延缓胃排空。本研究应用棕榈酸(palmitic acid,PA)诱导的HepG2细胞,观察Sirt1...
总的来说,研究结果表明,马尾藻是改善肥胖及其并发症的一个可行的药用选择。 Effects of low molecular weight polysaccharide fromSargassum thunbergiiagainst palmitic acid-induced intracellular lipid accumulation in 3T3-L1 adipocyte and...
参考文献 Ubiquitin-specific peptidase 10 ameliorates hepatic steatosis in nonalcoholic steatohepatitis model by restoring autophagic activity: Palmitic acid(PA) and vehicle were purchased from Kunchuang Co.Ltd.
HepG2 cells were cultured in vitro, and palmitic acid (PA) was used to construct the IR cell model. In the IR model, transfection of miR-124-3p or phosphoglucomutase 1 (PGM1) linked plasmids were transfected into HepG2 cells. RT-qPCR was used to determine the miR-12...
Palmitic acid was used to induce IR mode of HepG 2 cells in vitro . The effects of low-concentration ,medium-concentration and high-concentration (20,40, 80 mg/L) of TFB on the consumption of glucose were investigated. Using metformin as positive control ,the effects of low-concentration,...
Low molecular weight polysaccharides can be isolated fromSargassum thunbergii(LMPST) andin vitroexperiments were conducted to evaluate the inhibitory effects on lipids. Two natures of LMPST were attained fromS. thunbergiiand appraised their LMPST on palmitic acid (PA) induced lipid accretion in HepG...
METHODS :Using human hepatoma HepG 2 cells as research objects , 1 mmol/L palmitic acid and 2 mmol/L oleic acid were used to establish the IR-HepG 2 cell model. Using metformin hydrochloride as positive control ,the effects of low-concentration ,medium-concentration and high-concentration MGF...
基于上述实例,在体外研究中,技术人员为了模拟高甘油三酯和游离脂肪酸所致的脂毒性,通常需要添加高浓度的硬脂酸(Stearic acid),软脂酸(软脂酸,Palmitic acid),和/或油酸(Oleic acid),这是因为上述三种脂肪酸是存在于动物、植物及微生物中最常见的饱和/不饱和游离脂肪酸。正常情况下,人体血液中上述三种脂肪酸的浓度维...
ObjectiveTo investigate the mechanism of erythropoietin (EPO) on gluconeogenesis and glycogen synthesis in palmitic acid (PA)-induced HepG2 cells. MethodsHepG2 cells were cultured in medium with 250 μmol/L PA for 24 h , and treated with 5 or 10 U/ml EPO for 24 h. In addition, HepG...
Palmitic acid was used to induce IR mode of HepG 2 cells in vitro . The effects of low-concentration ,medium-concentration and high-concentration (20,40, 80 mg/L) of TFB on the consumption of glucose were investigated. Using metformin as positive control ,the effects of low-concentration,...