非酒精性脂肪性肝病(NAFLD)是以肝实质细胞脂肪蓄积和脂肪变性为特征的一种代谢紊乱性疾病。现有多数文献中记载了:胰高血糖素样肽-l(GLP-1)受体激动剂(Ra),如降糖药物利拉鲁肽(Lira)既能改善肝脂肪变性及胰岛素抵抗,还可抑制饱食中枢、延缓胃排空。本研究应用棕榈酸(palmitic acid,PA)诱导的HepG2细胞,观察Sirt1...
参考文献 Ubiquitin-specific peptidase 10 ameliorates hepatic steatosis in nonalcoholic steatohepatitis model by restoring autophagic activity: Palmitic acid(PA) and vehicle were purchased from Kunchuang Co.Ltd.
HepG2 cells were cultured in vitro, and palmitic acid (PA) was used to construct the IR cell model. In the IR model, transfection of miR-124-3p or phosphoglucomutase 1 (PGM1) linked plasmids were transfected into HepG2 cells. RT-qPCR was used to determine the miR-12...
总的来说,研究结果表明,马尾藻是改善肥胖及其并发症的一个可行的药用选择。 Effects of low molecular weight polysaccharide fromSargassum thunbergiiagainst palmitic acid-induced intracellular lipid accumulation in 3T3-L1 adipocyte and HepG2 cells Hyo-Geun Leea, D.P. Nagahawattaa, M.J.M.S. Kureraa,b...
Low molecular weight polysaccharides can be isolated fromSargassum thunbergii(LMPST) andin vitroexperiments were conducted to evaluate the inhibitory effects on lipids. Two natures of LMPST were attained fromS. thunbergiiand ...
Palmitic acid was used to induce IR mode of HepG 2 cells in vitro . The effects of low-concentration ,medium-concentration and high-concentration (20,40, 80 mg/L) of TFB on the consumption of glucose were investigated. Using metformin as positive control ,the effects of low-concentration,...
基于上述实例,在体外研究中,技术人员为了模拟高甘油三酯和游离脂肪酸所致的脂毒性,通常需要添加高浓度的硬脂酸(Stearic acid),软脂酸(软脂酸,Palmitic acid),和/或油酸(Oleic acid),这是因为上述三种脂肪酸是存在于动物、植物及微生物中最常见的饱和/不饱和游离脂肪酸。正常情况下,人体血液中上述三种脂肪酸的浓度维...
Palmitic acid was used to induce IR mode of HepG 2 cells in vitro . The effects of low-concentration ,medium-concentration and high-concentration (20,40, 80 mg/L) of TFB on the consumption of glucose were investigated. Using metformin as positive control ,the effects of low-concentration,...
目的比较饱和脂肪酸棕榈酸(palmitic acid,PA)和单不饱和脂肪酸油酸(oleic acid,OA)对HepG2细胞凋亡和自噬的影响.方法 PA和OA处理HepG2细胞,CCK-8检测脂肪酸的细胞毒性,caspase-3活性测定检测脂肪酸对细胞凋亡的影响,油红O染色检测细胞内脂质沉积,酶法检测细胞内甘油三酯的含量,Western blot检测自噬相关蛋白LC3-Ⅱ...
METHODS :Using human hepatoma HepG 2 cells as research objects , 1 mmol/L palmitic acid and 2 mmol/L oleic acid were used to establish the IR-HepG 2 cell model. Using metformin hydrochloride as positive control ,the effects of low-concentration ,medium-concentration and high-concentration MGF...