(The cells were stimulated in vitro with different concentrations of palmitic acid (PA, Kunchuang Biotechnology, China, KC001) coupled with control solvent (Kunchuang Biotechnology, China, KC001) for different periods, as indicated. Fatty acid-free solvent was used as a vehicle control for PA....
非酒精性脂肪性肝病(NAFLD)是以肝实质细胞脂肪蓄积和脂肪变性为特征的一种代谢紊乱性疾病。现有多数文献中记载了:胰高血糖素样肽-l(GLP-1)受体激动剂(Ra),如降糖药物利拉鲁肽(Lira)既能改善肝脂肪变性及胰岛素抵抗,还可抑制饱食中枢、延缓胃排空。本研究应用棕榈酸(palmitic acid,PA)诱导的HepG2细胞,观察Sirt1...
总的来说,研究结果表明,马尾藻是改善肥胖及其并发症的一个可行的药用选择。 Effects of low molecular weight polysaccharide fromSargassum thunbergiiagainst palmitic acid-induced intracellular lipid accumulation in 3T3-L1 adipocyte and...
HepG2 cells were cultured in vitro, and palmitic acid (PA) was used to construct the IR cell model. In the IR model, transfection of miR-124-3p or phosphoglucomutase 1 (PGM1) linked plasmids were transfected into HepG2 cells. RT-qPCR was used to determine the miR-1...
Palmitic acid was used to induce IR mode of HepG 2 cells in vitro . The effects of low-concentration ,medium-concentration and high-concentration (20,40, 80 mg/L) of TFB on the consumption of glucose were investigated. Using metformin as positive control ,the effects of low-concentration,...
Low molecular weight polysaccharides can be isolated fromSargassum thunbergii(LMPST) andin vitroexperiments were conducted to evaluate the inhibitory effects on lipids. Two natures of LMPST were attained fromS. thunbergiiand appraised their LMPST on palmitic acid (PA) induced lipid accretion in HepG...
基于上述实例,在体外研究中,技术人员为了模拟高甘油三酯和游离脂肪酸所致的脂毒性,通常需要添加高浓度的硬脂酸(Stearic acid),软脂酸(软脂酸,Palmitic acid),和/或油酸(Oleic acid),这是因为上述三种脂肪酸是存在于动物、植物及微生物中最常见的饱和/不饱和游离脂肪酸。正常情况下,人体血液中上述三种脂肪酸的浓度维...
Palmitic acid was used to induce IR mode of HepG 2 cells in vitro . The effects of low-concentration ,medium-concentration and high-concentration (20,40, 80 mg/L) of TFB on the consumption of glucose were investigated. Using metformin as positive control ,the effects of low-concentration,...
需要金币:*** 金币 (10金币=人民币1元)棕榈酸增加fatcd36表达和棕榈酰化修饰致hepg2细胞内脂质异常积聚-palmitic acid increases fat cd 36 expression and palmitoyl modification leads to abnormal lipid accumulation in hepg 2 cells.docx 关闭预览 想预览更多内容,点击免费在线预览全文 免费在线预览全文 棕榈...
目的:观察三七总皂苷(Panaxnotoginsengsaponins,PNS)对棕榈酸(Palmiticacid,PA)诱导的HepG2细胞炎症反应的作用,探讨PNS在抗炎作用中可能的机制。方法:体外培养HepG2细胞,根据实验要求设置对照组、PA处理组、PNS预保护组。采用MTT实验检测PA和PNS对HepG2细胞增殖的影响;油红O染色检测HepG2胞内脂质沉积;RealtimePCR检测...