结果转染siRNA后HepG2细胞IGF-Ⅰ受体表达下降。与放射组和干扰组比较,联合组细胞增殖率显著下降,PCNA mRNA的相对表达降低[(1.02±0.08)比(1.08±0.10)比(0.60±0.07)],G2/M期细胞比例增多[(20.3±0.3)%比(22.6±0.4)%比(34.7±0.5)%],差异有统计学意义(均P<0.05)。同时,联合组CDK1蛋白相对表达低于其他三...
α-1-抗凝乳蛋白酶、结合珠蛋白、铜蓝蛋白、纤溶酶原、补体C4、C3激活物、纤维蛋白原、α-1酸性糖蛋白、α-2-HS-糖蛋白、β-脂蛋白、视黄醇结合蛋白;表达胰岛素受体和胰岛素样生长因子IGFⅡ的受体;该细胞具有3-羟基-3-甲酰辅酶A还原酶和肝甘油三酯脂肪酶的活性。
摘要: 目的 探讨胰岛素样生长因子结合蛋白2(IGFBP2)对胰岛素样生长因子-1(IGF-1)诱导人源肝癌(HCC)细胞株HepG2细胞增殖、迁移和侵袭能力的影响及其机制.方法 选用15.6、31.2、62.5、125、250 ng/ml的IGFBP2与50 ng/ml的IGF-1联合刺激HepG2细胞.用CCK-8法检测细... 查看全部>> ...
HepG2细胞表达标志物包括胰岛素;胰岛素样生长因子II(IGF II)。HepG2细胞表现3-羟基-3-甲基戊二酰-CoA还原酶和肝甘油三酯脂肪酶活性,对葛兰素酮(氧化应激)的刺激表现为apoA-I mRNA表达减少、过氧化氢酶mRNA表达增加。没有证据表明HepG2细胞中存在乙型肝炎病毒基因组。除了基于原始出版物(PubMed:6248960)的所称的...
是一个合适的转染宿主；表达标志物包括胰岛素受体；胰岛素样生长因子 II（IGF II）受体。
The IGF2BP1 down-regulation by either miR-196b or IGF2BP1 siRNA led to an increase in apoptosis and a decrease in cell viability and proliferation in normal culture conditions. However, IGF2BP1 silencing did not modify the chemoresistance induced by hypoxia, probably because it is not the ...
Thus, combination of IGF1R and EGFR inhibition may be a promising novel treatment in HCC.%目的:探讨同时沉默胰岛素样生长因子1(IGF1)和表皮生长因子(EGF)二受体基因的抗瘤放疗增敏及机制.方法:分别构建靶向EGFR,IGF1R及两受体的小分子干扰RNA(siRNA-EGFR,siRNA-IGF1R,siRNA-EGFR& IGF1R),构建与任何基因...
Meanwhile, APC (10mg) offset IR-induced inhibition on the expressions of IGF-1R and IGF-1, the activation of AKT and STAT5, and the secretion of IGF-1 in HepG2 cells. More importantly, the reversal effect of APC on IR-induced alterations in HepG2 cells was counteracted b...
Hypoxia activates the IGF-1 expression through STAT5b in human HepG2 cells. Biochem Biophys Res Commun. 2007;358:733-8.Y.-H. Joung, M.-Y. Lee, E.-J. Lim et al., "Hypoxia activates the IGF-1 expression through STAT5b in human HepG2 cells," Biochemical and Biophysical Research ...
In the meanwhile, the expressions of CDK1 and CDK2 were decreased and the expression of Caspase-3 was increased in hypoxic HepG2 cells after IGF-1R knockdown (P<0.05). Conclusion: Interfering IGF-1R by siRNA inhibits the malignant biological behaviors...