Understand the metabolism of your compounds by using our hepatocyte stability assay to measure in vitro intrinsic clearance or to identify metabolites formed. The hepatocyte stability assay is one of our portfolio of in vitro ADME screening services. Cyprotex delivers consistent, high quality data with...
Cell proliferation was measured by a MTT assay (ATCC). Cells were plated at a density of 5 × 103cells per well in 96-well culture plates. After treatment, MTT solution was added to the culture medium (0.5 mmol/l) and incubated for 2 h at 37 °C with 5% CO2. Detergent...
In the early stages of drug discovery, the in vitro hepatic metabolic stability assay carries great importance as it provides a time- and cost-effective route for measuring the metabolic clearance in a high-throughput fashion and predicts in vivo hepatic clearance through in vitro-in vivo ...
Hepatocyte-derived Igκ interacts with ETFA and maintains the stability of the ETFA protein. A Scheme displaying the procedure used for identifying the interacting protein of Igκ through Co-IP assay and mass spectrometry. B Heatmap showing the potential binding proteins of Vκ4-1/Jκ3-Igκ...
S9a). Using the cell viability assay, we found that their growth rates were comparable (Additional file 1: Fig. S9b). In Fos KO organoids cultured with expansion medium, cholangiocyte markers (Sox9 and Spp1) were upregulated, while hepatocyte markers were barely detectable by qRT-PCR (...
(Fig.3h, i). Next, tumorsphere formation assay was applied to investigate whether CV hepatocytes were more like cancer stem/progenitor cells than PL hepatocytes after DEN injection. Most hepatocytes died within 48 h of seeding, but a population of spherical formations was observed 7 days ...
, alamar blue proliferation assay and live/dead staining. hemocompatible ba scaffolds supported the formation of high density hepatocyte clusters, facilitating cell-matrix and cell-cell interactions. blend scaffolds evinced enhanced liver-specific functions of cultured hepatocytes in terms of albumin ...
Eradication of malaria is difficult because of the ability of hypnozoite, the dormant liver-stage form of Plasmodium vivax, to cause relapse in patients. Research efforts to better understand the biology of P. vivax hypnozoite and design relapse preventi
Hepatocellular carcinoma (HCC) remains a global challenge due to its high morbidity and mortality rates as well as poor response to treatment. The communication between tumor-derived elements and stroma plays a critical role in facilitating cancer progre
This review summarizes the latest findings on human hepatocytes isolation, cryopreservation, culturing, as well as application in the evaluation of metabolic stability, metabolite profiling, hepatic uptake and efflux, metabolic drug-drug interactions, and drug toxicity. The use of hepatocyte to evaluate ...