The main parameters were set as follows: retention time tolerance, 0.2 min; actual mass tolerance, 5 ppm; signal intensity tolerance, 30%; ratio of signal to noise ratio, 3; and minimum inten- sity 100,000. Then, the molecular formula was predicted via additive ions, molecular ion peaks,...
Human embryonic stem cell lines (hESCs) are from the inner cell mass of blastocysts. This process requires the damage to the embryo and thus has given rise to ethical problem [29]. Thus, IPSCs are known to have overcome these issues. IPSCs are reprogrammed back into an embryonic- like ...
nutrients Article Regressive Effect of Myricetin on Hepatic Steatosis in Mice Fed a High-Fat Diet Shu-Fang Xia 1,2,*, Guo-Wei Le 2, Peng Wang 3, Yu-Yu Qiu 1, Yu-Yu Jiang 1 and Xue Tang 2 1 Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China; yuyuqiu1102@aliyun.com...
2.3. Animal Sample Analysis Plasma free fatty acids (FFAs), were determined using Gas Chromatography-Mass Spectrometer (GC-MS) as described in our previous study [21]. Plasma ALT and insulin were detected using ALT test Kit (Wako, Osaka, Japan, 996-10801) or mouse insulin ELISA Kit (...
The remaining mass includes cholangiocyte parenchyma and the non-parenchymal cells, including sinusoidal endothelial, stromal, Kupffer, and stellate cells. The liver has many important biological functions, such as detoxification, modification and excretion of exogenous and endogenous substances, the ...
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4.6. Capillary Electrophoresis Mass Spectrometry NP and FNP (10mg each) were suspended in five hundred microliters of methanol con- taining 8 µM of internal standards (methionine sulfone for cation and camphor 10-sulfonic acid for anion analyses), 500 µL of chloroform and 200 µL of ...
The sections were blocked in 10% normal horse serum and incubated with a primary antibody (1:100 diluted) against Wilms' Tumor Protein 1 (WT1) from Boster Biological Technology (Pleasanton, CA, USA) overnight at 4 ◦C. The sections were incubated with biotinylated secondary antibody (Vector...
Introduction Bee pollen is a mass of pollen grains in the hive after storage and fermentation. Because bees collect the most abundant nutrients on a variety of stamens, bee pollen is also known as "essence of flowers" [1,2]. Bee pollen has been widely used as a nutraceutical due to its...
Administration of IOE to high fat diet (HFD)-fed mice inhibited body and WAT mass gain, attenuated fasting hyperglycemia and dyslipidemia. The obesity suppression was associated with reductions in expression of adipogenic proteins, such as C/EBPα and PPARγ, increases in expression of lipolytic ...