The main parameters were set as follows: retention time tolerance, 0.2 min; actual mass tolerance, 5 ppm; signal intensity tolerance, 30%; ratio of signal to noise ratio, 3; and minimum inten- sity 100,000. Then, the molecular formula was predicted via additive ions, molecular ion peaks,...
4.6. Capillary Electrophoresis Mass Spectrometry NP and FNP (10mg each) were suspended in five hundred microliters of methanol con- taining 8 µM of internal standards (methionine sulfone for cation and camphor 10-sulfonic acid for anion analyses), 500 µL of chloroform and 200 µL of ...
Hepatocytes, the main parenchyma of the liver, comprise almost 80% of the adult liver's total mass [41]. The remaining mass includes cholangiocyte parenchyma and the non-parenchymal cells, including sinusoidal endothelial, stromal, Kupffer, and stellate cells. The liver has many important ...
2.3. Animal Sample Analysis Plasma free fatty acids (FFAs), were determined using Gas Chromatography-Mass Spectrometer (GC-MS) as described in our previous study [21]. Plasma ALT and insulin were detected using ALT test Kit (Wako, Osaka, Japan, 996-10801) or mouse insulin ELISA Kit (...
The IHC reaction was visualized using EnVision + System-HRP polymer kit as a secondary antibody (1:1000, Code K4001, Dako, Denmark). 2.8. Imaging of Reactive Oxygen Species Frozen hepatic tissues were stained with dihydroethidium (DHE, 37291, Sigma, St. Louis, MO, USA) to visualize the ...