administration of mRNA encoding human FGF21 proteins. The efficacy of mRNA was assessed following 2-weeks repeated s.c. dosing in diet-induced obese (DIO), mice which resulted in marked decreases in body weight, plasma insulin levels, and hepatic steatosis. Pharmacokinetic/pharmacodynamic (PK/PD)...
14]. Energy production, primarily in the form of ATP, is controlled by mitochondria that link oxidative respiration with the metabolism of nutrients, such as carbohydrates, fatty acids, and amino acids. Two major steps, oxidation of NADH or FADH2and phosphorylation...
The overexpression of FGF21 in mice increases systemic glucocorticoid levels, suppresses physical activity and alters circadian behavior. However, mice lacking the gene encoding β-Klotho (Klb) in the SCN are refractory to these effects, suggesting that the diverse physiologic and pharmacologic actions ...
Specifically, the results demonstrate that hepatic FGF21 is a critical molecular intermediate between diet, PPARα, and the physiological regulation of hepatic and systemic lipid metabolism. Results FGF21 Is a Signaling Molecule Induced in the Ketotic State Microarray analysis was performed to identify...
In Crtc1 KO liver, we found that expression of Pgc1α and genes regulating the mitochondrial and peroxisomal fatty acid oxidation pathways9 upon overnight fasting was substantially reduced compared to wild-type liver (Fig. 2j). Additionally, fibroblast growth factor 21 (Fgf21), a fasting-...
FGF15, and then anti-Mel/anti-FGF15 antibody and 50 ul streptavidin-HRP were added respectively, and the plate was covered with a sealing membrane and incubated at 37 °C for 60 min. The plate was washed five times, and the chromogenic solution and stop solution were added in sequence, ...
Results: Alcohol intervention altered the levels of 31 of 72 and 14 of 72 markers in the systemic and hepatic circulation. All changes observed in the hepatic circulation were also identified in the systemic circulation after 180 min. Only FGF21 and IL6 were increased after alcohol int...
FGF21, Fibroblast Growth Factor 21; VLDL, Very Low-Density Lipoproteins; CE, Cholesteryl Ester; HDL, High-Density Lipoproteins; LDL, Low-Density Lipoproteins; SR-BI, Scavenger receptor class B type 1; WAT, White Adipose Tissue; HSL, Hormon-Sensitive Lipase; FAT/CD36; Fatty Acid Translocase ...
In parallel, it has been shown that transcription factors such as FGF, HGF, Wnt, BMP, RA, and TGFβ to correlate with effectors influencing liver development. Therefore, it has been expected that transferring and integration, overexpression or knockdown of one or more than one transcription ...
BA re-uptake by enterocytes stimulates FGF-19 release into the portal blood, which inhibits BA synthesis. (B) Free fatty acids (FFA) derive from lipolysis in adipose tissue and are actively taken up by various FA transporters under the control of insulin (Ins) and nuclear receptor signaling....