HEPARIN-DEPENDENT PLATELET ANTIBODYClotting of vascular access in hemodialysis patients remains a significant problem. The routine use of heparin to anticoagulate the blood during hemodialysis has been helpful in increasing the life span of vascular access in these patients. However, the use of heparin...
Pathogenic anti-PF4 antibody was assessed by serotonin-release assay. Heparin-dependent anti-PF4 antibodies were detected in 11 SLE (9%) and 2 primary ITP (3%) patients, but at much lower levels than in HIT patients. In serotonin-release assays, only the HIT sera induced platelet activation ...
We test all cardiac surgery patients who experience thrombocytopenia, resistance to heparin, or have complications of increased bleeding or thrombosis with platelet aggregation assay for heparin-dependent antiplatelet antibody (HAAb). Due to the morbidity and mortality associated with reexposure to heparin...
patients with heparin-associated thrombocytopenia (HAT) were evaluated by a quantitative enzyme-linked immunosorbent assay (ELISA) to detect heparin-dependent serum platelet-bindable immunoglobulin (S-PBIg) and by Western blotting and immunoprecipitation to investigate the specificity of the antibody binding...
Kelton JG, Sheridan D, Brain H, et al. Clinical usefulness of testing for a heparin-dependent platelet aggregating factor in patients with suspected heparin associated thrombocytopenia. J Lab Clin Med 1984; 103: 606–12 PubMedCASGoogle Scholar ...
Similar results were obtained using 14 C-serotonin release instead of platelet aggregation as a measure of platelet activation. These findings suggest that (1) the reaction of the heparin-dependent antibody with platelets and heparin is ... BH Chong,I Fawaz,CN Chesterman,... - 《British Journal...
Type II HIT also differs from other immune thrombocytopenias in its pathogenic mechanism. Intravascular, heparin-dependent platelet aggregation is a more likely explanation for the thrombocytopenia than the phagocytosis of antibody-laden platelets by the reticuloendothelial system. ...
Antiplatelet antibody was not detected in the patient globulin fractions prepared from serum collected within one week after heparin withdrawal by use of the platelet factor 3 availability technic. When the studies were repeated with modifications to detect heparin-dependent antiplatelet antibodies, ...
Heparin-induced thrombocytopenia (HIT) is a serious antibody-mediated reaction. HIT occurs in patients treated with heparin and is due to the development of antibodies to a platelet Factor 4-heparin complex that induce in vivo platelet aggregation. HIT may progress to the development of venous and...
topeniaoccursfromplateletconsumption ExcessPF4bindstoglycosaminoglycansonendothelialcellsformingim- munecomplexes Anti-PF4antibodiesbindtotheendothelialcells,causingantibody- mediatedendothelialinjury Thrombidevelopatthesitesofvascularinjuryanddisseminatedintravas- ...