Apo(a) in normal human serum can be detected at normal size with the same monoclonal antibody. Moreover, purchased monoclonal antibody to Lp(a) had been prepared to be highly specific to human apo(a) and to be not reactive to plasminogen. According to our results, it was suggested that ...
Assay of Reactive Oxygen Species (ROS) Generation The DCFH-DA method was used to determined the effects of 1–3 on ROS generation as reported68. In this method, the stable non-fluorescent DCFH-DA was hydrolyzed by esterases in cells to non-fluorescent DCFH, which was rapidly oxidized by ROS...
If utilizing alternate cell type, excessive primary/secondary antibody or dye concentrations for cell type – titrate dilutions to optimize signal. Additional wash steps after the primary/secondary antibody incubations may help decrease background fluorescence due to non- specific binding. Check for ...
For cytoskeletal proteins, either a rabbit anti-b-tubulin (diluted 1:10 in 3% BSA in PBS and revealed by a Texas red-conjugated anti-rabbit-IgG antibody) for 1 h or a 10 mM FITC-conjugated phalloidin for 20 min were used. All the incubations were performed at room tem- perature. ...
Moreover, unlike the previous study [45], where the antibody used was only capable of measuring TG2-L expression, the antibody used in the current study proved to be cross-reactive to both TG2-L and TG2-S isoforms, confirming that TG2-S levels were also unchanged during the development...
Figure 1. HepG2 cells’ viability upon 24 h exposure to paraquat (PQ) (A) and to Ginkgo biloba leaf extract (B). Cell viability was assessed using Alamar Blue assay (see Methods), data are expressed as % of control (Cont.; non-treated cells) and are shown as mean ± S.D (n =...
Cell lysate samples were diluted 1:1 with PBS. To the enzyme-linked immunosorbent assay strip wells (MyBioSource) coated with polyclonal anti-ceramide antibody, 100 μL of each sample was added, ten μL balance solution was dispensed, and 50 μL of the conjugate was added to each well. ...