For cytoskeletal proteins, either a rabbit anti-b-tubulin (diluted 1:10 in 3% BSA in PBS and revealed by a Texas red-conjugated anti-rabbit-IgG antibody) for 1 h or a 10 mM FITC-conjugated phalloidin for 20 min were used. All the incubations were performed at room tem- perature. ...
Figure 1. HepG2 cells’ viability upon 24 h exposure to paraquat (PQ) (A) and to Ginkgo biloba leaf extract (B). Cell viability was assessed using Alamar Blue assay (see Methods), data are expressed as % of control (Cont.; non-treated cells) and are shown as mean ± S.D (n =...