B-TPGS 2.09 1 2.09 14.32 0.0069 AB 0.672 1 0.672 4.62 0.068 A2 0.223 1 0.223 1.53 0.2557 B2 0.571 1 0.571 3.92 0.0881 Residual 1.02 7 0.145 Lack of Fit 0.485 3 0.161 1.21 0.4131 not significant Pure Error 0.534 4 0.133 Cor Total 9.94 12 Table 5. Characteristics of the Optim...
Li H M,Chen J,Xiong C M, et al.Apoptosis induction by the total flavonoids from Arachniodes exilis in HepG2 cells through reactive oxygen species- mediated mitochondrial dysfunction involving MAPK activation [ J] .Evidence- based complementary and alternative medicine, 2014, ( 2 ) : 1 - 11...
Effect of normoxia/hypoxia and melatonin treatments on VEGF mRNA levels (B), VEGF cell protein levels (C) and VEGF secreted to the culture media (D). HUVECs tube formation assay (E). Tube formation index (S.CORE) (F). Data are expressed as a percentage of mean values±s.e.m. of ...
Notes: (A, B) Two independent TAZ shRNAs were transfected into HepG2 liver cancer cells. Western blotting was used to observe the knockdown efficiency. (C) The MTT assay was used for cell viability detection. Two independent TAZ shRNAs were transfected into HepG2 liver can...
(B) Total TG2 mRNA expression was measured using RT-PCR. (C) Transaminase activity was measured using a TG2-specific colorimetric assay kit. TG2 activity is the enzymatic activity of TG2 to perform protein–protein cross linking, transamination, and deamidation of proteins. Error bars are ...
were examined. Tris-Tricine–PAGE revealed prominent detectable bands between 10 and 100 kDa. LC–MS/MS identified 150–180 proteins and peptides in the protein probes, and GO analysis demonstrated a distinct enrichment of proteins associated with “extracellular space” and “proteasome core complex...
BDE-47 was also positively associated with the KRAS signaling and negatively with the reactive oxygen species pathway, UV response UP, and allograft rejection. Figure 3. Heatmaps of combined DEGs and DEPs associated with the significantly enriched Hallmark gene sets (a) mTORC1 signaling, (b) ...
2.11. Intracellular ROS Level Measurement Intracellular ROS production was measured using dihydroethidine (DHE) dye (Assay Kit Reactive Oxygen Species (ab236206), Abcam, Cambridge, UK) in live cells, which is selective for superoxide anion and hydrogen peroxide. Briefly, following incubation with ...
In total, four cultured conditions were studied: (A) steatotic HepG2 cells exposed to basal medium containing no fatty acids; (B) steatotic HepG2 cells exposed to basal medium containing no fatty acids but supplemented with the defatting cocktail; (C) steatotic HepG2 cells exposed to the...
Briefly, cDNA was converted from1 µg isolated total RNA using a cDNA synthesis kit (Bio-Rad). The cDNA libraries were amplified while using the universal and index-specific primer. The purified library was checked for quality and yield using a DNA high sensitivity chip and a Bioanalyzer (...