In the case of APAP, the hepatotoxicity of the drug is known to be associated with N-acetyl-p-benzoquinoneimine (NAPQI), a reactive metabolite of CYP2E133, and unrepaired damage to DNA34,35. APAP is metabolized into NAPQI by CYP2E1, and the metabolized NAPQI exerts the cytotoxic effect....
(total cholesterol,TC)水平,DCFH-DA 检测药物 对细胞内活性氧 ( reactive oxygen species , ROS ) 生成的影响 , 采用试剂盒检测细胞内氧化应激关键指标丙二醛 (malondialdehyde,MDA),总谷胱甘肽(glutathione,GSH)水平以及超氧化物歧化酶(superoxide dismutase,SOD)活性; 采用 Western blotting 检测过氧化物酶体增殖物...
For each analysis, 20 μL of the sample was injected into the column, maintained at room temperature, with a total run time of 10 min. To prepare the standard DAS solution, 10 mg of DAS was accurately weighed and transferred to a 10 mL volumetric flask. After dissolving in 5 mL of ...
A total of 254 mRNAs were identified as up- or down-regulated over 1.5- fold in Akbu-LAAO-treated HepG2 cells in comparison with control HepG2 cells. Among these targeting genes, we focused on the molecules involved in TGF-β signal pathway. Gene-gene interaction network analysis ...
The membranes were incubated with primary antibodies overnight at 4 °C after blocking using a 5% nonfat milk solution for 1 h. Next, secondary antibody was used to incubate the blots under room temperature for 1 h. Finally, reactive protein was evaluated using Image Lab analysis software ...
monounsaturated fatty acids ROS: reactive oxygen species TOFA: 5-(tetradecyloxy)-2-furoic acid References Tejeda-Maldonado J, Garcia-Juarez I, Aguirre-Valadez J, Gonzalez-Aguirre A, Vilatoba-Chapa M, Armengol-Alonso A, Escobar-Penagos F, Torre A, Sanchez-Avila JF, Carrillo-Perez DL. ...
In an in vitro study, the mitochondrial reactive oxygen species levels (mROS) are highly correlated with the therapeutic sensitivity of liver cancer to sulforaphane. Moreover, the mitochondrial energy metabolism and mitochondrial calcium homeostasis drastically control the viability of ...
The amount of IL-6 in the portal vein was associated with the amount of C-reactive protein derived from the liver.11 Although the association between the VAT–liver interac- tion and inflammation has been established, the pathophysiolo- gical mechanisms by which adipose tissue may induce a ...
A total of 21 proteins were identified as differentially expressed proteins and these 21 proteins were all up-regulated by Arg supplementation. Six amino acid metabolism-related proteins, mostly metabolic enzymes, showed differential expressions. Intriguingly, Ingenuity Pathway Analysis (IPA) based pathway...
The present disclosure relates to a novel hepatocyte-like cell progenitor and/or a novel hepatocyte-like cell derived via definitive endoderm from human blastocyst-derived stem (hBS) cells, to a method for the preparation of such cells and to the potential use of such cells in, e.g., pharma...