(total cholesterol,TC)水平,DCFH-DA 检测药物 对细胞内活性氧 ( reactive oxygen species , ROS ) 生成的影响 , 采用试剂盒检测细胞内氧化应激关键指标丙二醛 (malondialdehyde,MDA),总谷胱甘肽(glutathione,GSH)水平以及超氧化物歧化酶(superoxide dismutase,SOD)活性; 采用 Western blotting 检测过氧化物酶体增殖物...
Yan et al. have stated that having high levels of reactive oxygen species (ROS) inside a cell can speed up the cell cycle being stopped which then leads to cell death. Thus, it is very important to look for ROS in HepG2 cells that have been planted with different levels of tiny ...
known as non-alcoholic fatty liver disease, is strongly associated with a proinflammatory and prothrombotic state.29 Levels of the proinflammatory factors C-reactive protein and IL-6, as well as the prothrombotic factors fibrinogen and PAI-1, were shown to increase with more severe degrees of hepa...
A total of 21 proteins were identified as differentially expressed proteins and these 21 proteins were all up-regulated by Arg supplementation. Six amino acid metabolism-related proteins, mostly metabolic enzymes, showed differential expressions. Intriguingly, Ingenuity Pathway Analysis (IPA) based pathway...
The membranes were incubated with primary antibodies overnight at 4 °C after blocking using a 5% nonfat milk solution for 1 h. Next, secondary antibody was used to incubate the blots under room temperature for 1 h. Finally, reactive protein was evaluated using Image Lab analysis software ...
Apoptosis Induction by the Total Flavonoids from Arachniodes exilis in HepG2 Cells through Reactive Oxygen Species-Mediated Mitochondrial Dysfunction Involving MAPK Activation. Evid-Based Compl Alt. 2014;2014(5):906941.Li HM;Chen J;Xiong CM;.Apoptosis Induction by the total flavonoids from Arachni...
a The population of apoptotic cells was investigated by double staining of Annexin V/PI, determined by flow cytometry, and depicted as representative flow cytometry scatterplots. b, c The histogram shows percentages of cell viability and total apoptosis. d The percentages of the CPT-1 activity ...
The present disclosure relates to a novel hepatocyte-like cell progenitor and/or a novel hepatocyte-like cell derived via definitive endoderm from human blastocyst-derived stem (hBS) cells, to a method for the preparation of such cells and to the potential use of such cells in, e.g., pharma...
After treatment, total RNA was obtained by using a Trizol reagent (Applied Biosystems, Carlsbad, CA, USA) and quantified by spectrophotometry (Nanodrop 1000; Thermo Scientific, Waltham, MA, USA). Residual genomic DNA was removed by incubating RNA with RQ1 RNase-free DNase (Promega, Madison, ...
(GSH) in the cell is consumed, leading to the inactivation of GPX4, which ultimately leads to lipid peroxidation and the production of a large number of reactive oxygen species, constituting the main mechanism leading to cell ferroptosis [11,12,13]. As an essential part of the Xc- system,...