HAV: IgM antibody is diagnostic for acute infection. IgG positive but IgM negative indicates past exposure. HBV: Acute infection is indicated by the presence of surface antigen, IgM core antibody, envelope antigen, and viral load. However, there is also a “window period” during which the sur...
Clinicians should pay attention to ANA patterns and titer during the interpretation of this test.Key Points Defining the clinical relevance of antinuclear antibody (ANA) using indirect immunofluorescence assay in the context of diseases can be an important tool for the clinician in the diagnostic work...
This genotype has been associated with more aggressive liver disease as well, but it is unclear if this results from a longer duration of infection among people infected with this genotype. Genotypic differences may affect usefulness of diagnostic immunoassays for HCV antibody which are based on ...
OBJECTIVES: To verify the clinical impact of autoantibody screening on the diagnosis of Systemic Lupus Erythematosus (SLE) and other autoimmune diseases through LR measurement when HEp-2 cells are used for screening or investigation into anti-Ds-DNA antibody, showing the importance of parameters such...
The ANA Hep Screen ELISA assay allows for collective and simultaneous screening for the autoantibodies of major significance in one microwell, and effectively eliminates the need for individual interpretation that is inherent in IFA technology. 上海玉博生物技术有限公司在为生命科学领域提供丰富的产品与信息...
Prior to N-glycome analysis, the effi- ciency of the treatment was checked by immunofluorescence (IF) using anti-5mC antibody (Fig. 1a), and by pyrosequencing of the LINE-1 elements (Fig. 1b). Pyrosequencing analysis of the LINE-1 elements showed a significant decrease (ranging from...
In the indirect immunofluorescence antibody test, microscope glass slides are coated with HEp-2 cells, and human serum is incubated with the HEp-2 cells to allow serum autoantibodies to react with autoantigens in HEp-2 cells. The binding of autoantibodies is detected by fluorescently tagged anti...
PARP cleavage was analyzed for respective treatments by a specific antibody by western blotting (Panel B and D). Full size image Figure 8 TUDCA inhibits UV-B irradiation and PBA induced apoptosis: TUDCA (2 mM) pretreated HepG2 cells were treated with UV-B irradiation for 60 seconds and...
After incubating the PVDF membrane at room temperature with a secondary antibody at 1:6000 for 1 h, the membrane was eluted with TBST 3 times for 10 min each. An ECL kit was used for exposure, and grey value analysis software was used for the protein grey analysis. Each experiment was ...
H) Immunofluorescence staining images of pre-coated recellularized liver ECM – HepG2 in red (A1AT – specific antibody staining), HAEC in green (CD31 – specific antibody staining); original magnification 63×; nuclei – DAPI in blue. (For interpretation of the references to color in this ...