ReactionrateThe purpose of this study was to verify the concept of non-equilibrium facilitated oxygen diffusion. This work succeeds our previous study, where facilitated oxygen diffusion by hemoglobin was measured at conditions of chemical equilibrium, and which yielded diffusion coefficients of hemoglobin...
Structure and oxygen affinity of crystalline desArg141 alpha human hemoglobin A in the T state. The correlation of a protein structure determined crystallographically to its functional properties determined in solution can be an extremely complex prob... JS Kavanaugh,DR Chafin,A Arnone,... - 《Jou...
We describe experiments which elucidate the reaction mechanism of electrochemical oxygen reduction reaction (ORR) on a glassy carbon electrode modified with hemoglobin in an aqueous environment but with the hemoglobin physically separated from the electrode by a porous Nafion layer. Catalytic amplification ...
2c, if the solution is not deaerated, only the cathodic peak, but not a pair of redox curves, appears at around −130 mV, with the current of 3.6 μA. The reaction mechanism is as follows [19], [20], [21]:HbFe(III)+e-→HbFe(II)HbFe(II)+O2→HbFe(II)⋯O2HbFe(II)...
destruction associated with a bad reaction to a blood transfusion. A disease that is defined by too little hemoglobin in the blood is known as anemia. There are various types of anemia. WHAT IS MEAN CORPUSCULAR HEMOGLOBIN? Mean corpuscular hemoglobin concentration(abbreviated as MCHC) is an estima...
Both fast and slow pseudo-first order oxygen combination rate constants varied linearly with oxygen concentration between 16 and 1300 muM. A first order slow relaxation was also noted which was linearly dependent on heme concentration and inversely dependent on oxygen concentration. This reaction has ...
parasitica Hb, similar reduction appears to be associated with endothermic conformational changes that accompany the oxygenation reaction. 展开 关键词: hemoglobin oxygen binding enthalpy deep-sea fish hydrothermal vent anguillid zoarcid Thermarces cerberus Symenchelis parasitica ...
A possible reaction mechanism of the pantetheine conjugation catalyzed by Hb is as follows: (i) the oxygen of oxygenated ferrous heme (Fe2+-O2) of Hb is reduced by pantetheine (in the case of ferric heme [Fe3+], it is reduced to ferrous [Fe2+] by thiol, and then the resulting ...
The valence-hybrid thus formed was reacted with oxygen and with carbon monoxide. Kinetics of the reactions were studied. The effects of pH, inositol hexaphosphate, and temperature on these reactions were examined. The kinetics of the ligation of O2 and CO were used to characterize the affinity ...
For example, the flavohemoglobin from Escherichia coli or yeast was found to detoxify NO through an oxygen-dependent NO dioxygenation reaction or an oxygen-independent NO reduction reaction, thereby protecting the cells against nitrosative stress [1,11–14]. On the other hand, the Hb found in ...