Crystals were formed in 1.8 mol/L potassium phosphate buffer, pH 7.4, at 30 degrees C and were counted in several time intervals with a hematocytometer. The hemoglobin composition of Millipore-isolated crystals and supernatant were also analyzed. Under the conditions selected, 100% HbS formed ...
Peripheral blood may show moderate amounts of target cells and intracellular crystals (5-30%)[24, 25] . Hemoglobin C Disease As stated before, hemolytic anemia may be mild to moderate in severity. Markers of hemolysis include increased LDH, reticulocyte count, and direct bilirubin[1] . ...
1.24). Occasionally there are classic Hb C crystals (see discussion under Hemoglobin CC Disease). Some cells show irregularly concentrated hemoglobin. S/β-thalassemia differs from SS disease in that there is microcytosis, fewer sickled cells, and more target cells. Ancillary Studies Hb variant ...
Intraerythrocytic hemoglobin crystals in sickle cell-hemoglobin C disease. Blood 1965;25:218 A/223.Diggs LaB, A (1965) Intraerythrocytic hemoglobin crystals... LW Diggs,A Bell - 《Blood》 被引量: 39发表: 1965年 Dyserythropoiesis, Sideroblasts/Siderocytes and Hemoglobin Crystallization in a Dog...
hemoglobin A 2的构成为α 2 Aδ 2 A 2 或α 2 Aδ 2,它在成人血内通常只有少量。已报道过多种血红蛋白,如血红蛋白S,C,D,E,G,H,I,J,K,L,M,N,Q,Norfolk,Barts等,它们的电泳活动度和特性均不同,参看hemoglobinopathy项下。由于生化技术的改进,已发现了更多的血红蛋白类型,也制定了一些命名的标准。
Ferryl hemoglobin was made by incubation of oxyhemoglobin with a 10:1 ratio of H2O2 to heme for 5 h at 37°C. After oxidation, ferryl hemoglobin was dialyzed against saline (three times for 1 h at 4°C) and concentrated with Amicon Ultra centrifugal filter tubes (10,000 MWCO, Merck...
Apart from being the direct cause of quite a few cases of near-fatal poisoning, carbon monoxide is a very common noxious agent functioning as an additional factor in human disease. The extent of the possible role of carbon monoxide in human pathology is demonstrated by the following data from...
Diagnosis is made by the (a) presence of familial erythrocytosis (polycythemia), (b) exclusion of other causes of polycythemia (polycythemia vera, cyanotic heart disease), (c) high red cell mass, (d) high arterial O2 saturation, (e) a markedly LEFT shifted O2 dissociation curve. ...
Hb S. In Hb AC trait and Hb CC disease, glycosylated Hb C will also elute in the “S” window. In these conditions, Hb A2′ will remain undetected. Conversely,sickle cellpatients on chronic transfusion protocol or recent, efficient RBC exchange may have a very small percentage of Hb S,...
Because of its insolubility, crystals of HbC can sometimes be seen in peripheral blood smears from homozygous individuals. As a result of the coincidental distribution of the genes for HbS and HbC, heterozygotes for both hemoglobins are not uncommon. HbSC disease has a severity intermediate ...