Then, the cells were centrifuged and the red blood cells in the spleen were lysed by red blood cell lysate (Sigma-Aldrich, USA) for 5 min. The CD8+ T cells (OT-1 cells) of spleen were separated by EasySepTM mouse CD8+ T Cell Isolation Kit (Stemcell, Canada). Afterwards, OT-1 ...
1A), and this result was corroborated by western blot (control: 0.176 ± 0.035 vs hemin: 1.476 ± 0.385; p = 0.0007) (Fig. 1B). We generated a heatmap with the 6377 proteins detected in seven vehicle- and hemin-treated biological replicates (Supplementary Fig. S1C). ...
Satisfactory ResultBlood SerumHuman BloodA novel mimetic enzyme immunoassay to determine 伪-1-fetoprotein (AFP) in solution was developed. Hemin, a horseradish peroxidase substitute, was used as a labelling reagent to catalyze the reaction of p-hydroxyphenylacetic (HPA) and hydrogen peroxide in ...
2F) test under 77 K were carried out and showed that the Brunauer-Emmett-Teller (BET) surface area of Cu-hemin MOFs decreased from 17.00 to 3.08 m2 g−1 after the loading of GOD. The result further confirmed that GOD molecules were loaded in the pores of Cu-hemin MOFs. The...
Hemin is a naturally derived chloride of heme from animal blood and can be used as an effective promoter of heme oxygenase-1 (HO-1), which is broken down by HO-1 to produce carbon monoxide (CO), biliverdin (BV), and ferrous ions (Fe2+)28,29. Its structure is a Fe3+complexed prot...
peptides and amino acids that are transported into cytoplasm and used for cell growth15. Antimalarial drugs could interfere with hemoglobin digestion during blood stages of malarial life-cycle33,68,74. Chloroquine inhibits lysosomal functions34and protein degradation in trophozoite-infected erythrocytes75...
Hemin is a naturally derived chloride of heme from animal blood and can be used as an effective promoter of heme oxygenase-1 (HO-1), which is broken down by HO-1 to produce carbon monoxide (CO), biliverdin (BV), and ferrous ions (Fe2+)28,29. Its structure is a Fe3+complexed prot...
that associated with reduced risk for kidney injury in patients undergoing cardiopulmonary bypass35,36. Since all patients had similarly elevated blood hepcidin concentrations37, the differentially increased amount of hepcidin in urine could be explained by local renal production. Indeed, hepcidin is synth...
Hemin is a coordination complex of iron (oxidation state: III) (Figure 1b) [60], isolated from bovine blood (Russia). Figure 1. Structural formulas of PHB (a) and hemin (b). 2.2. Preparation of the Electrospun Materials Polymer nanofibrous materials were obtained by electrospinning (ES) ...
The development of innovative fibrous materials with valuable multifunctional properties based on biodegradable polymers and modifying additives presents a challenging direction for modern materials science and environmental safety. In this work, high-performance composite fibrous materials based on semicrystalline...