Heme is an essential cofactor for many biological processes in aerobic organisms, which can synthesize it de novo through a conserved pathway. Trypanosoma cruzi , the etiological agent of Chagas disease, as well as other trypanosomatids relevant to human health, are heme auxotrophs, meaning they ...
Subsequently, collinearity analysis was performed using MCScanX58, with parameters set to ‘-s 4 -e 1e-5’, meaning that at least four genes were required to be considered a collinear block, with the e-value threshold set to 1e-5. As shown in Fig. 4, a high degree of collinearity ...
Berghuis & Brayer, et al,. “Oxidation state dependent conformational changes in cytochrome-c,” Journal of Molecular Biology 223(4), pp. 959-976 (1992). Brown, et al., “Aggregation of ferrihaems. Dimerization and protolytic equilibria of protoferrihaem and deuteroferrihaem in aqueous solut...
Interestingly, a U-shaped association between Hb and CVD incidence or outcome has been observed, meaning both anemia and high Hb levels are implicated [51, 52]. As we did not have anemic individuals in the current study population (3% individuals had Hb < 130 g/L and only 2 ...
Efficient electron transfer occurs when the free energy for the electron to be transferred from donor to acceptor is negative meaning that the reduction potential of an intermediary must be fine-tuned to be in between those of the donor and the acceptor. Heme iron reduction potentials can be ...
proteins, while also underscoring the voids in our understanding of chemistry which need to be filled. 2. Redox communication 2.1. A conversation between cofactors: redox communication Metalloproteins and metalloenzymes are efficient tools in biology using metals to achieve both electron transfer and ...
Regardless, the complete association and dissociation phases are used to calculate the KD, meaning the reported values represent a “composite” affinity of both phases. In all cases, the χ2 values (how well the models fit the actual data) were low (<2) and rigorous (see “Experimental ...
When functionalized magnetic beads immobilized with tartaric acid interacted with heme, that would be adsorbed on the magnetic beads, meaning only one tartaric acid coordinated with heme along an axis perpendicular to the porphyrin ring. Subsequently, the immobilized heme reacted with tartaric acid and...
Subject Area: Biochemistry, Genetics and Molecular Biology More specific subject area: Preservation of enzymes in freeze-dried whole-cells for biotransformations Method name: A simple and efficient method for lyophilization of recombinant E. coli JM109 (DE3) whole-cells harboring active Rieske non-hem...
There are several types of plastids, but the fundamental type is the chloroplast (from the Greek chloros, meaning “green,” and plastes, meaning “the one who forms”). Specialized functions in plant cells have evolved, such as photosynthesis and production of phytochrome chromophore precursors ...