A helicase is defined as an enzyme that catalyzes the separation of double-stranded nucleic acids into single strands in an ATP-dependent reaction, playing a crucial role in various DNA-related processes such as replication, repair, and transcription. ...
It has been proposed that the MCM helicase may act as a double-strand DNA translocase that pumps duplex DNA into the double hexamer in a manner analogous to that of the SV40 large T antigen or the bacterial RuvAB Holliday junction branch-migration enzyme (Figure 1). Under this scenario, ...
HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51 Article Open access 22 December 2021 C16orf72/HAPSTR1/TAPR1 functions with BRCA1/Senataxin to modulate replication-associated R-loops and confer resistance to PARP disruption Article Open access 17 August 2023 XRN2 inter...
Nanoscale transport through nanopores and live-cell membranes plays a vital role in both key biological processes as well as biosensing and DNA sequencing. Active translocation of DNA through these nanopores usually needs enzyme assistance. Here we present a nanopore derived from truncated helicase E1 ...
A GUCT domain has also been identified in human RNA DEAD-box protein 21 (DDX50; RHII/Guβ), a multifunctional enzyme with RNA-unwinding and RNA-folding activities (Valdez et al. 1997). Bioinformatic analyses of the GUCT domain have shown the presence of the RNA recognition motif (RRM) ...
Movement through the DNA helix produces positive supercoils in front of the polymerase, making the DNA helix more difficult to open and negative supercoils behind the enzyme facilitating DNA helix opening [47]. Accumulation of negative supercoils in the NTS region behind the promoter site may ...
A significant shift of the titration curves toward higher total [DnaB]T, in the presence of poly(dA), indicates efficient competition between the two nucleic acids for the enzyme. At the same value of the relative fluorescence increase, the total average binding density, (Σνi)S, on the ...
Overexpression of alanine substitution mutants of conserved amino acids in motifs I, IV, V and VI and the RNA polymerase basic domain results in increased mtDNA copy number as is observed with overexpression of the wild type enzyme. In contrast, overexpression of three N-terminal mutants W282L,...
The Mpox pandemic, caused by the Mpox virus (or monkeypox virus, MPXV), has gained global attention. The D5 protein, a putative helicase-primase found in MPXV, plays a vital role in viral replication and genome uncoating. Here we determined multiple cryo-EM structures of full-length hex...
(G21), which is the first base within the folded G4 that would be encountered by the 3′–5′ translocating RecQ enzyme, was found sequestered in a guanine-specific pocket (GSP) on the surface of RecQ. The GSP forms hydrogen bonds with the guanine base using the sidechain hydroxyl and ...