Both the AMPPNP and the single-stranded segment of the oligonucleotide are bound by the two ‘core’ fragments of the α–β domains, but in spatially disparate regions; the oligonucleotide is cradled by From structure to mechanism When we survey the structural information that has emerged on ...
2. Helicase structure, mechanism, and classification Helicases catalyze the hydrolysis of nucleotide triphosphates (typically ATP) and convert this chemical energy into mechanical energy that enables the separation of base-paired strands of nucleic acids [53], [54]. One example of this is the “me...
Structure and mechanism of helicases and nucleic acid translocases. Annu Rev Biochem 2007; 76:23-50; PMID:17506634; http://dx.doi. org/10.1146/annurev.biochem.76.052305.115300Singleton MR, Dillingham MS, Wigley DB. Structure and mechanism of helicases and nucleic acid translocases. Annu ...
Structure and Mechanisms of SF1 DNA Helicases Abstract Superfamily I is a large and diverse group of monomeric and dimeric helicases defined by a set of conserved sequence motifs. Members of this class are involved in essential processes in both DNA and RNA metabolism in all organisms. In addi...
UvrD, a model for non-hexameric Superfamily 1 helicases, utilizes ATP hydrolysis to translocate stepwise along single-stranded DNA and unwind the duplex. Previous estimates of its step size have been indirect, and a consensus on its stepping mechanism is lacking. To dissect the mechanism underlying...
B. Structure and mechanism of helicases and nucleic acid translocases. Annu. Rev. Biochem. 76, 23–50 (2007). Article CAS PubMed Google Scholar Umate, P., Tuteja, N. & Tuteja, R. Genome-wide comprehensive analysis of human helicases. Commun. Integr. Biol. 4, 118–137 (2011). ...
Singleton, M. R., Dillingham, M. S. & Wigley, D. B. Structure and mechanism of helicases and nucleic acid translocases.Annu. Rev. Biochem.76, 23–50 (2007). ArticleCASPubMedGoogle Scholar Umate, P., Tuteja, N. & Tuteja, R. Genome-wide comprehensive analysis of human helicases....
(2002). Helicase structure and mechanism. Curr. Opin. Struct. Biol. 12: 123–133, https://doi.org/10.1016/s0959-440x(02)00298-1.Search in Google Scholar Chakrabarti, S., Jayachandran, U., Bonneau, F., Fiorini, F., Basquin, C., Domcke, S., Le Hir, H., and Conti, E. (...
By using single-particle cryo-EM of an active CMG on the forked DNA with a dual biotin-streptavidin block, we solved the first structure of helicase in complex with a DNA fork. The DNA duplex stem penetrates into the central channel of the NTD tier and the unwound leading ssDNA traverses...
B. Structure and mechanism of helicases and nucleic acid translocases. Ann. Rev. Biochem. 76, 23–50 (2007). CAS PubMed Google Scholar Cordin, O., Banroques, J., Tanner, N. K. & Linder, P. The DEAD-box protein family of RNA helicases. Gene 367, 17–37 (2006). CAS PubMed...