Noncoding RNAs related to the hedgehog pathway in cancer. The five main components of the Hh signaling are Hh, Ptch, Smo, Sufu, and Gli. The ncRNAs introduced in this review include circRNA, microRNA, lncRNA and snRNA. This diagram briefly describes the interaction between ncRNAs and five c...
Venn diagrams were generated using Venn Diagram R package and statistical analysis of intersections is derived from SuperExactTest R package. For PDOX experiments, the Shapiro–Wilk test was used to validate the assumption of normality. Statistical significance was then determined using Kruskal–Wallis...
6 weeks old female balb/c mice were maintained in proper light /dark cycle of 12/12 h at Institute of Life Sciences, Bhubaneswar, India. All the animal work and the experimental protocol were approved by institutional animal ethical committee (IAEC, Institute of Life Sciences, Bhubaneswar, ...
We demonstrate here a plausible SCIENTIFIC REPORTS | 5 : 7915 | DOI: 10.1038/srep07915 1 www.nature.com/scientificreports Figure 1 | (A) Schematic diagram of a model to explain TMZ-response in GBM neoplastic cells on the perspective of clonal heterogeneity. (B) The experimental design for ...
The HIF pathway plays a role in PK cell injury from prednisolone administration inL7Cre;Vhl(fl/fl) mice.aRight, schematic diagram showing transgenic mouse breeding to target Purkinje cells inL7Cre;Vhl(fl/fl) animals. Left, schematic of cerebellar circuit highlighting Purkinje-specific Cre recombina...
Effects of cell cycle phase were calculated and regressed out using the CellCycleScoring function. The four samples were merged and then aligned using the MultiCCA function, using the top 1000 genes that were highly variable in at least two datasets. To identify cell clusters, the top 15 ...
(i) Diagram showing FLAG-NPHP4 deletion constructs used inh. (j) Binding assay of immuno-purified GFP (negative control) or GFP-KIF13B-C2861-1,000mixed with bacterially purified GST fused to the central C2 domain of NPHP4 (GST-N4-C22650-839). (k) IFM analysis ofNphp4+/+andNphp4...
Figure 1. Schematic diagram of Smo-driven canonical and non-canonical Hh signaling pathways. (A) In the canonical Hh signaling, when Hh ligands are absent, Ptch1 exerts a repressive activity on Smo. Gli factors are sequestered by Sufu and phosphorylated by PKA, CK1, and GSK-3β, and then...
Figure 1. Schematic diagram of Smo-driven canonical and non-canonical Hh signaling pathways. (A) In the canonical Hh signaling, when Hh ligands are absent, Ptch1 exerts a repressive activity on Smo. Gli factors are sequestered by Sufu and phosphorylated by PKA, CK1, and GSK-3β, and then...