To quantify the degree of sorting in cardioids with mixed progenitors (labelled by using either WTC: H2B-GFP or WTC: LMNB1-RFP line), we analyzed the cross-section of the cardioids. To ensure comprehensive coverage of all cells in the organoids, we superimposed the GFP and RFP signals us...
b A diagram displaying the electrical responsiveness of the different heart chambers to similar regional optical pacing across the different mouse genetic lines. c Comparison of effective refractory periods (ERP) determined by RV epicardial optical programmed stimulation in hearts from three optogenetic ...
Using c-kit antibody, we frequently detected cells in wild-type hearts that were dually labelled with c-kit and PECAM (Supplementary Fig. 10a4,d4,g2 and Supplementary Fig. 11a,f,h,i). In c-kitH2B-tdTomato/þ animals, c-kit antibody immunoreactivity co-localized with c-kitH2B-td...
The visual diagram is adopted from Chromosome Analysis Suite (Affymetrix Inc.,). From right to left the diagram presents copy number coordinates, the patient’s probe distribution, paternal and maternal probes distributions, OMIM genes, miRNAs, all SNP and copy number probes in the region, and c...
FIG. 4 is a schematic functional block diagram illustrating an implantable device for delivering ETC signals to the heart using the method of timing of the non excitatory signal, in accordance with a preferred embodiment of the present invention; ...
Violin plots consisting of cell numbers of ten or fewer are not shown and are labelled as ‘NA’ in those cases. Reporting summary Further information on research design is available in the Nature Portfolio Reporting Summary linked to this article....
After ischaemia reperfusion injury, we found that Rhodamine 6G-labelled leucocytes could easily be identified within the vessel. Indeed, individual leucocytes could be clearly seen rolling along the endothelium (Fig. 5f). In Fig. 5f, we show four consecutive frames obtained at a speed of 15 ...
By 48 hpf, the looped zebrafish heart appears S-shaped and undergoes more subtle changes until both chambers are morphologically distinguishable19,21. Using our adaptive prospective optical gating software, we have shown that we can directly witness these changes in 3D time-lapse video (Fig. 3a;...
While we labelled spots by their cell cycle, we did not regress out the cell cycle features for the purposes of clustering. Marker genes for cross-capture cell types were determined using FindAllMarkers and used for pathway enrichment within enrichR (v3.0) and clusterProfiler (v3.18.1). Cell...
The presence of endocardial damage or a differential pressure between two chambers (either between atrium and ventricle, or left and right chambers in case of intracardiac shunt) can facilitate their formation. Valve vegetations tend to be found on the low-pressure surface. They are, therefore,...