Genes specific for platelet–red blood cells were not selected. To validate the final target gene list, we tested whether we could transcriptionally rederive the cell populations by cluster analyses using only the 238 target genes. To this end, we reduced the scRNA-seq dataset to only the 238...
We provide the following evidence: (1) Exosome secretion from EAT is increased in rapid atrial pacing canines, with labeled EAT exosomes distributed in the hippocampus. Microglial cells in the hippocampal tissue of rapid atrial pacing canines are significantly activated. The use of exosome inhibitors...
Subsequently, isolated plasma and blood cells were carefully packaged, labeled, and stored in a cryogenic freezer at − 80 ° C. In this study, blood cells were used for single nucleotide polymorphism (SNP) detection because of their high sensitivity and reliability. Questionnaires were ...
Heart disease involves irreversible myocardial injury that leads to high morbidity and mortality rates. Numerous cell-based cardiac in vitro models have been proposed as complementary approaches to non-clinical animal research. However, most of these app
Each sample's 8 bp barcode was labeled PCR production, and all the libraries were pooled. After that, cluster generation and hybridization of sequencing primer were performed. Base incorporation was carried out on the Miseq sequencing system (Illumina, Inc., San Diego, CA) following the ...
The heart is an organ which is in continuous motion with the never ending task of pumping blood, as well as being in a constant state of mechanical and metabolic flux. It is not just a pump, but it can adapt itself by carefully regulating the rate and force ofcardiac contractionsby respo...
The heart features four types of valves which regulate the flow of blood through the heart. These valves have been clearly shown in the labeled diagram of the heart. They permit blood flow in one direction only, and prevent backflow of blood. The four types of valves are: ...
Measurement of myocardial blood flow Myocardial blood flow was measured by using method of fluorescent microspheres perfusion69. Briefly, 500000 microspheres of 15μm in diameter that labeled with red fluorescence (Molecular Probes, Invitrogen, CA, USA) were injected into left ventricular over more tha...
1e,f). In addition to the findings from scRNA-seq, flow cytometry analysis confirmed the parallel changes in cardiac immune cells during sepsis progression (Extended Data Fig. 2d,e). Fig. 1: Single-cell atlas of cardiac immune cells during sepsis progression. a, Schematic diagram showing ...
Fig. 1. Wiggers diagram: pressure, volume and ECG records [3]. Plethysmography. Plethysmographic methods are based on the local modification of blood volume occurring during systole and diastole. On each cardiac cycle, during systolic phase, blood is ejected through the aortic valve in the circ...