In both cell lines changes in doubling times occurred: in HT29 an increase, and in HCT116 a decrease. Cell survival in response to hypoxia and to DNA damage differed strikingly in the two cell lines. The HT29 hypoxia-conditioned cells were more resistant than the parental line to a 24...
Cell cycle- analysis of chalcone7fin HCT116 cells. (A) control HCT 116 cell line; (B) HCT116 cells treated with chalcone7f. Full size image Figure 13 DNA contents in each cycle of tested chalcone7fand control HCT116cell line. Full size image Figure 14 Cell cycle analysis of chalcone7ftr...
Increased levels of GFP-BAX were unable to stimulate higher levels of cell death. Examination of GFP-BAX distribution before and after indomethacin treatment indicated that BAX protein did not form aggregates when present at sub-lethal concentrations. Conclusion Within the limitations of this ...
The interactions between ABZ and microtubule-binding agents, paclitaxel, vinblastine, colchicine, and 2-methoxyestradiol were characterized using median effect analysis method in HCT-116 colorectal cancer cells and DU145 prostate cancer cell line. The mechanism underlying the synergistic interaction related ...
Apoptosis is a form of programmed cell death that is required for the maintenance of tissue homeostasis by counterbalancing cell proliferation and eliminating damaged or transformed cells. Apoptosis occurs as a result of caspase activation which causes the collapse of cellular infrastructure through ...
Flow cytometry analysis of mode of cell death in Gemini-Chr NPs treated colorectal cancer cells. Annexin V-FITC/propidium iodide (PI) histograms were obtained from analysis of colorectal cancer HCT-116 cells incubated with 40 and 60 µM of Gemini-Chr NPs for 24 h Full size image Bax, Bcl...
Over 40 cells of each cell line were used for the analysis. Statistical analysis was performed using a two-tail Student's t-test, where P = 0.001. 24 h in the medium containing 0, 10 and 100 ng/ml of doxycycline (DOX), respectively. The levels of Bcl-2 were measured by immuno...
In addition to HCT116 cells, we also determined the WMJ-S-001's effects on growth of another two colorectal cancer cell lines, HT29 and Colo205 cells. HT29 is a p53 mutant cell line49 while Colo205 cell retains functional p5350. As shown in Fig. 7a, WMJ-S-001 significantly ...
Full size image Integrin-β1 KO in a breast carcinoma cell line was previously reported to inhibit the expression of EMT-related molecules9. Therefore, we predicted that ERO1α KO would influence the expression of EMT-related molecules through the effect on integrin modification under hypoxia, and...
Full size image COP blockaded the survival signal pathways of HCT-116 cells We explored whether the COP could inhibit the cell survival and growth of HCT-116 cells by the PI3-kinase-Akt pathway and the ERK pathway. Treatment on HCT-116 cells with stated doses of COP for 24 h down-reg...