As shown inFig. 1A, in HCT-116 cells, COP exhibited cell cytotoxicity at a high rate (IC50was 27.13 μg/mL vs 282.2 μg/mL in FHC). Besides, COP (2.5 μg/mL) decreased the survival rate of cells time-dependently (Fig. 1B). However, COP treatment induced relatively low...
Cell survival in response to hypoxia and to DNA damage differed strikingly in the two cell lines. The HT29 hypoxia-conditioned cells were more resistant than the parental line to a 24 h hypoxic challenge, while those from HCT116 surprisingly were more sensitive. Sensitivity to cisplatin in ...
Increased levels of GFP-BAX were unable to stimulate higher levels of cell death. Examination of GFP-BAX distribution before and after indomethacin treatment indicated that BAX protein did not form aggregates when present at sub-lethal concentrations. Conclusion Within the limitations of this ...
The interactions between ABZ and microtubule-binding agents, paclitaxel, vinblastine, colchicine, and 2-methoxyestradiol were characterized using median effect analysis method in HCT-116 colorectal cancer cells and DU145 prostate cancer cell line. The mechanism underlying the synergistic interaction related ...
2.3. Cell culture The HCT 116 cell line (ATCC, USA) was grown in McCoy’s 5A medium (Sigma) supplemented with 10 % heat-inactivated fetal bovine serum (FBS, Sigma), 1% penicillin/streptomycin (100 μg/mL, PAA Laboratories, Austria), 1 % amphoteric B (250 μg/mL, PAA Laboratories, ...
Annexin V-FITC/propidium iodide (PI) histograms were obtained from analysis of colorectal cancer HCT-116 cells incubated with 40 and 60 µM of Gemini-Chr NPs for 24 h Full size image Bax, Bcl-2, and P53 genes expression Real-time PCR was used to further confirm the mode of cell ...
Full size image Flow cytometer analysis Chalcone7fwas selected for more investigations, in attempts to find its molecular action on cancer cells. It illustrated high and very interesting cytotoxicity toward the tested colon cancer cell line (HCT116) as mentioned in the above MTT assay data (Table...
Full size image Integrin-β1 KO in a breast carcinoma cell line was previously reported to inhibit the expression of EMT-related molecules9. Therefore, we predicted that ERO1α KO would influence the expression of EMT-related molecules through the effect on integrin modification under hypoxia, and...
tion. To investigate the effect of the H1047R mutation on cell morphology and actin cytoskeleton structure, we used cell lines harboring either WT or mutant (MUT; H1047R) p110α of PI3K, which were generated by asymmetric deletion of the PIK3CA allele from the CRC parental cell line HCT116...
Injection of the colorectal cancer cell line HCT116 below the skin of the mouse led to a fast-growing tumor with poor differentiation (Supplementary Figure S3). When the tumor achieved a measurable volume (50–300 mm3), oxaliplatin was administered, typically one to several times a week for ...