The protein was transfer printed to a polyvinylidene difluoride membrane and then incubated with a 5% bovine serum albumin solution to block nonspecific binding. Subsequently, the membrane was subjected to incubation with targeted primary antibodies at a temperature of 4 ℃ for the duration of 8–10...
GraphPad Prism 8.0 software (San Diego, CA, USA) or IBM SPSS 24.0 software (IBM, New York, NY, USA) was used for statistical analysis. Unpaired Student’st-test was used for comparisons of two groups. Comparisons between more than two groups were performed using ANOVA with Tukey’s or Bo...
Han et al. found that adhesive junction- associated protein 1 (AJAP1) oversubscription restrained tumor initiation and pulmonary metastasis in HCC and that heterotopic expressed of AJAP1 reduced the mul- tiplication, oncogenicity and transfer of HCC cells [40]. Finally, Zha et al. determined ...
Androgen receptor variant 7 (AR-V7), an AR isoform with a truncated ligand-binding domain, functions as a transcription factor in an androgen-independent manner. AR-V7 is expressed in a subpopulation of hepatocellular carcinoma (HCC), however, its role(s) in this cancer is undefined. In this...
Hence, we examined if exosomes released from cancer cells could transfer miR-200b-3p to endothelial cells. Exosomes were isolated from HCC cell lines and expression levels of miR-200b-3p in exosomes were measured. The expression levels of miR-200b-3p in the exosomes were high in Hep3B ...
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(DEN), CD4+T cells have been found to prevent tumor initiation and mediate the clearance of premalignant hepatocytes10. In humans, adoptive transfer of tumor-specific CD4+T cells caused a complete tumor eradication in a patient bearing cholangiocarcinoma, another primary liver cancer11. Furthermore...
YIPF2 acts as a GDF (GDI-displacement factor) to catalyze the dissociation of Rab-GDI complexes, and to enable transfer of Rab5/22a from GDI onto distinct endosomes or ER-Golgi membranes. Activated Rab5 and Rab22a localize to distinct compartments and regulate CD147 transport from endocytosis,...
Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates. Nat Genet. 2009;41:753–61. 27. Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, et al. CRISPR- mediated direct mutation of cancer genes in the ...
Exosomal transfer of TUC339 to neighbor tumor cells promotes HCC cell proliferation and reduces cell adhesion to extracellular matrix. In this case, we ask whether HCC-derived exosomal lncRNAs can be transferred to neighbor macrophages and how this intercellular communication would influence macrophage ...