aHBV-DNA 荧光定量试剂盒由上海科华生物工程股份有限公司提供;日本OLYMPUS IX-71荧光倒置显微镜;德国罗氏公司LightCycler 480 实时荧光定量PCR 仪;日本OLYMPUS 公司AU-2700全自动生化分析仪。 The HBV-DNA fluorescence quota reagent box provides by the Shanghai branch China bio-engineering limited liability company...
Quantitative Detection of Hepatitis B Virus DNA in Two International Reference Plasma Preparations Quantitative detection of hepatitis B virus (HBV) in serum or plasma is of significance for monitoring of therapy and establishment of the prognosis of the... KH Heermann,WH Gerlich,M Chudy,... ...
The present invention also relates to a kit for detecting HBV-DNA comprising said primers and probes. Specifically, the present invention is characterized in that said HBV is either an adr sub-type or an adw sub-type.CHOI, HYUN ILPARK, HYUNG KEUN...
ChemicalBook 为您提供Hepatitis B virus nucleic acid (HBV DNA) qualitative and quantitative national reference|乙型肝炎病毒核酸(HBV DNA)定性及定量国家参考品的供应商,生产企业,生产厂家。
乙型肝炎病毒荧光PCR检测试剂盒(HBV)是基于荧光染料检测的即用型 PCR 试剂盒,可以对靶片段进行实时定量PCR 分析(quantitative PCR、qPCR)。产品含有经过优化的缓冲液组分、Taq DNA聚合酶、dNTPs、MgCl2及新型荧光染料 DNAgreen 等所有实时定量 PCR 所需要的成分,用户只需加入模板和引物即可进行实时定量 PCR 反应和 HRM...
该文作者采用自动的、原型定量的(血清)HBV RNA检测方法(prototype quantitative HBV RNA assay),对未接受和接受NUC治疗的慢性HBV感染患者的循环HBV RNA(cirB-RNA)水平,以评估 cirB-RNA 浓度与血液中和肝细胞中的、与cccDNA 转录活性相关的其他病毒标物之间的关系,以评估其作为一种新的无创、可靠、可重复的、用于监...
3)客户尽量不要提供DNA或RNA样品。 4)样本保存于液氮或干冰,也可以保存于Trizol液中。 实时荧光定量PCR(quantitative real-time PCR,qPCR)是指在PCR反应体系中加入荧光基团,利用荧光信号积累实时监测整个PCR进程,*通过标准曲线对未知模板进行定量分析的方法。实时荧光定量PCR的化学原理包括探针类和非探针类两类,探针类...
Florescence quantitative PCRObjective To explore the relation between the quantity of Hepatitis B virus DNA(HBV-DNA) and the HBV serum markers(HBV-M)... WU Minyi,L Chen,LI Qinmei,... - 《Clinical Medical & Engineering》 被引量: 3发表: 2011年 Exploring the relationship between hepatitis B ...
COMPRISING THE SAME A QUANTITATIVE METHOD FOR HBV-DNA, A PRIMER AND PROBE FOR DETECTING HBV-DNA, AND A DETECTING KIT COMPRISING THE SAMEA QUANTITATIVE METHOD FOR HBV-DNA, A PRIMER AND PROBE FOR DETECTING HBV-DNA, AND A DETECTING KIT COMPRISING THE SAMEThe present invention relates to a ...
(1)提取样本中的DNA; (2)构建标准曲线:使用特异性扩增五种人源病毒DNA的引物和探针组合对五种人源病 毒的不同给定浓度的DNA标准品进行qPCR检测,并用检测结果制作标准曲线,拟合线性方 程; (3)样品DNA检测:对样本DNA进行qPCR检测,并根据检测数据判断检测结果; ...