PCR analysis of gene pyramids for the presence target allele(s) (cropped gels displayed). 1. DNA amplification ofGm1alleles using the primer RM444. 2. DNA amplification ofGm4alleles using the primer RM547. 3. DNA amplification ofSub1allele using the primer SUB1BC2. 4. DNA amplification of...
Mouse having mutations including (i) a mutation in both alleles of protein kinase, DNA-activated, catalytic polypeptide (Prkdc) gene, (ii) a mutation in both alleles of the gene encoding for the interleukin-2 receptor (IL-2)-gamma -chain, (iii) a mutation in at least one allele of the...
two amino acids which are identical and in the sme relative positions and there should be a number, i.e. at least two and preferably at least three amino acids, at corresponding positions, which are not identical but have similar reactivities. In general there should be fewer than 20 ...
Mouse having mutations including (i) a mutation in both alleles of protein kinase, DNA-activated, catalytic polypeptide (Prkdc) gene, (ii) a mutation in both alleles of the gene encoding for the interleukin-2 receptor (IL-2)-gamma -chain, (iii) a mutation in at least one allele of the...
light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene; and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region, where the ...
A preferred condition is whether the peptide has a high probability of being bound with high affinity to the MHC molecule of the alleles of the given host and in the competitive context of its protein of origin while at the same time displaying a specific motif for recognition by a T-cell...
Thus, the 20S core is identical at its two ends. The x-ray structure of the archaebacterial proteasome has recently been resolved and was shown to contain a narrow pore in each α ring, and a large central cavity formed by the β rings. Accordingly, the central cavity is not exposed ...
Meldrum, “Automation for Genomics, Part Two: Sequencers, Microarrays, and Future Trends”, Genome Research, 10:1288-1303, 2000. Miyagishi M, and Taira K. (2002). U6-promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nature...
1. Method for diagnosing a person having multiple sclerosis (MS) or being at risk of developing MS, comprising the following steps: - detecting the presence or absence of wild type SCF-Apoptosis-Response Gene- (wt-SARG-1-) protein and/or nucleic acids encoding wt-SARG-1 in a sample of ...
Allele. An “allele” is any of one or more alternative form of a gene, all of which relate to one trait or characteristic. In a diploid cell or organism, the two alleles of a given gene occupy corresponding loci on a pair of homologous chromosomes. Amplicon. A piece of DNA formed as...