What if I want to target some other gene or use a different cell line? For the most frequently requested edits we have around 3000 knockout and knock-in cancer related models in many common cell lines such as DLD1, MCF10A and HCT116. If you want to use one of our 10 most popular ...
Over the past few years, the human near-haploid cell line HAP1 has widely been consolidated as one of the favorite cell line models for functional genetic studies. Its rapid turnover coupled with the fact that only one allele needs to be modified in order to express the subsequent desired ...
Current models for the synthetic lethal interaction between PARPi and HR centre on the disruption of recombination-based repair mechanisms, most notably during DNA replication. For example, PARPi result in the accumulation of unrepaired SSBs, or trap PARP1 and PARP2 at sites of DNA damage. Colli...
This third scenario, an Archaeal origin of fusexins, poses new challenges to both sex origins and eukaryogenesis models. Discovery of the Asgard superphylum55and the recent cultivation of one of its members56support eukaryogenesis scenarios where populations of bacteria and archaea lived in syntrop...
The scaffold was designed considering four design models namely the hollow cube, dumbbell, dual cone and honeycomb models with 62–67% of porosity. Post FE simulation analysis and design optimization using Pugh's matrix revealed that the honeycomb model was superior considering the mass (g), ...
that can be mobilized by a conjugative-like, cell fusion-dependent mechanism. Fig. 6: Environmental distribution of Fsx1s. aArchaeal fusexins unrooted phylogeny, environmental details, and trimeric models66based on the Fsx1 X-ray structure (PDB 7P4L [https://www.rcsb.org/structure/7P4L], ...
Structure determination by molecular replacement with AlphaFold2 models. Multiple attempts to experimentally determine the structure of Fsx1E using a variety of heavy atoms failed, probably because the high-salt mother liquor com- position hindered heavy atom binding. Because molecular replacement (MR) ...