verified the successful construction of the recombinant vector pcDNA3.1 (+)-CLIC1.The cell line Hca-P that steadily expressing CLIC1 gene was constructed. RT-PCR assay and ELISA showed that the 基金项目：国家自然科学基金面上项目(No：30772468)。通信作者：唐建武 E-mail:Jwtang_53@yahoo.cn ...
Our previous study (Oncotarget 2016; 7:46) demonstrated that the over-expression of sulfatase-1 in murine hepatocarcinoma Hca-F cell line (a murine HCC cell with lymph node metastatic [LNM] rate of >75%) downregulates mesothelin and leads to reduction in lymphatic metastasis, both in vitro ...
HCA2 expression was reported to be upregulated in murine macrophage cell lines ANA-1 and RAW264.7 by stimulation of IFN-γ in combination with TNF-α or LPS alone [20]. We therefore examined the capacity of IL-6 and IL-1β to induce HCA2 in mouse macrophages.Figure 1shows that unstimul...
【摘要】Objective To design small hairpin RNA (shRNA) targeting Annexin All (ANXA11) , to transfect mouse hepatoma Hca — P with Annexin All (ANXA11) , to obtain the Hca — P cell line with Anxal 1 stable knock — down and to establish material basis for future study. Methods Two ...