Hairy root transformationLiquiritinGuUGT1GuUGT10Genetic transformation is a fundamental tool in molecular biology research of medicinal plants. Tailoring transgenic technologies to each distinct medicinal plant would necessitate a substantial investment of time and effort. Here, we present a simple hairy ...
5). For evaluating larger proteins, in size range of NPR1, or those forming biochemical complexes as in CRISPR–Cas9, we suggest using the genetic-based hairy root transformation approach (Fig. 2 and Supplementary Fig. 4). Caution must also be exercised to ensure that the small peptides ...
4.1.3Development of hairy-like root In addition, development of a fast growing hairyroot systemhas not been known or performed yet in marine biotechnology of brownmacroalgae. However, transformation of tissues from terrestrial plants with the “natural genetic engineer”Agrobacterium rhizogenes, has bee...
While in some plant species, including soybean, genetic transformation is still recalcitrant and time-consuming, hampering the high-throughput functional analysis of soybean genes. Thus we pursue to develop a rapid, simple, and highly efficient hairy root system induced by Agrobacterium rhizogenes (A...
Genetic transformation of Echinacea purpurea with Agrobacterium rhizogenes and bioactive ingredient analysis in transformed cultures Colloids and Surfaces B: Biointerfaces 2006; 53:101–104. CAS Google Scholar Liu CZ, Abbasi BH, Gao M et al. Caffeic acid derivatives production by hairy root cultures ...
Root - inductionofPuerarialobatab y A g robacterium rhizo g eneR1000andthe actors ffectin g GeneticTransformation YuShuhong , LiLing ! , LiuChuanfei !! , PanRuichi ! ( CollegeofLifeScience , ~ebeiNormal niversity , Shijiazhuang050016 ) ( ! DepartmentofBiology , SouthChinaNormal niversity , ...
D. High-efficiency agrobacterium rhizogenes-mediated genetic transformation in Artemisia vulgaris: Hairy root production and essential oil analysis. Ind. Crop. Prod. 44, 643–652. https://doi.org/10.1016/j.indcrop.2012.09.007 (2013). Article CAS Google Scholar Gangopadhyay, M., Sircar, D., ...
During the past decade, the molecular mechanisms of crown gall and hairy root development have been elucidated in considerable detail. It now appears that
7.3.6Genetic Manipulation in Hairy Root Culture for Secondary Metabolite Production A. tumefaciensandA. rhizogenesinfected root furnish a promising platform for thegeneticmanipulation of plant cells. As discussedA. rhizogenes-mediated transformation of plants can be used in a similar manner to that used...
hairy root cultures. Plant Growth Regulation, 34, 339–345. Article Google Scholar Banerjee, S., Naqvil, A. A., Mandalt, S., & Ahuja, P. S. (1994). Transformation of Withania somnifera (L) Dunal by Agrobacterium rhizogenes: infectivity and phytochemical studies. Phytotherapy Research, 8...