Hairy rootProtocolSoybeanTransformationSoybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean ...
method. Then, four the expression vectors were transformed intoAgrobacterium tumefaciensstrain K599 for soybean hairy root transformation using hypocotyl transformation. After incubation about 2 weeks, the positive hairy root was identified using hand-held laser gun and collected into 50-mL centrifuge ...
while thenopLmutant was the negative control.bProximity Labeling (PL) assay for biotinylation of NFR5 in soybean hairy roots. GFP-TbID or REM1a-GFP-TbID fusion proteins were co-expressed with GmNFR5-FLAG in
Also, the soybean hairy root transient transformation system has been developed and used to study SCN resistance [33,67,76,90]. The early work on the functional analysis of SCN-resistance genes was done by Cook et al. [33], who used gene silencing in soybean hairy roots to show that ...
Soybean hairy root transformation and rhizobial inoculation Seeds of soybean cv. "Tianlong 1" were surface steri- lized by placing 150 seeds in 15 × 100 mm Petri dishes in a single layer. The plates were placed inside a 1000-mL beaker with 200 mL of commercial bleach. Ten ...
While in some plant species, including soybean, genetic transformation is still recalcitrant and time-consuming, hampering the high-throughput functional analysis of soybean genes. Thus we pursue to develop a rapid, simple, and highly efficient hairy root system induced by Agrobacterium rhizogenes (A...
The effector Hg16B09 was functionally characterized via soybean hairy root transformation, and the results verified that Hg16B09 enhances the susceptibility of soybean to SCN, so it is a virulent SCN effector (Hu et al. 2019). Recently, by single-nematode sequencing, 48 differentially expressed ...
The effector Hg16B09 was functionally characterized via soybean hairy root transformation, and the results verified that Hg16B09 enhances the susceptibility of soybean to SCN, so it is a virulent SCN effector (Hu et al.2019). Recently, by single-nematode sequencing, 48 differentially expressed gen...
The soybean hairy root transformation and salt treatments were performed as previously described57 with some modifications. Surface-sterilized soybean seeds were germinated on germination medium (15 mg l−1 NaH2PO4·H2O, 1 mM CaCl2·2H2O, 25 mM KNO3, 1 mM (NH4)2SO4, 1 mM MgSO4...
4A). The gene editing efficiency of each gRNA was verified by soybean hairy root transformation. We performed stable soybean transformation and obtained multiple T0 lines for each gRNA construct. DNA sequencing of the transgenic offspring identified a series of homozygous single or double mutants, ...