it remains largely unknown about how mucosal virome as a community regulates intestinal inflammation in humans. The ileum, the terminal part of the small bowel, is the primary site of CD infliction where various obligate and facultative anaerobic bacteria...
(Leiden, The Netherlands) using the NEXTflex 16S V4 Amplicon-Seq Kit (BiooScientific, Austin, TX) and the Illumina 2500 system (rapid mode, paired-end, 250 bp; Illumina, San Diego, CA, USA). Raw sequencing data are available in the European Nucleotide Archive (http://www.ebi.ac.uk/...
GQD). All P values in the GO enrichment analysis were adjusted for multiple testing using the BH method. Adjusted P < 0.05 was considered significant. Dot size represents the ratio of the number of DEGs to the number of genes in the corresponding entry. Dots are color-coded according to ...
For C3 testing, brain tissues were homogenized on ice in the PTR buffer (supplied in the kit). The supernatant was collected for ELISA detection after the homogenate was centrifuged at 13,000 g for 20 min at 4 °C and the protein concentration was determined by the BCA protein ...
was no or insufficient DNA contamination resulting from the kit to be of significance to the subsequent analysis of the metagenomic data. Libraries were sequenced by NGS Illumina sequencing to a depth of 10 Gbp/sample by Novogene, UK. Per-sample read numbers are shown in Supplementary Fig. S6...
Fatty liver hemorrhage syndrome (FLHS) becomes one of the most major factors resulting in the laying hen death for caged egg production. This study aimed to investigate the therapeutic effects of Lactiplantibacillus plantarum (Lp. plantarum) FRT4 on FLHS
Quality control was performed on the resulting FastQ files using FastQC software (https://www.bioinformatics.babraham.ac.uk/projects/fastqc), and bioinformatics analyses were conducted by using the Quantitative Insights into Microbial Ecology (QIIME 1.9.1) software package [33] with the subsampled ...
Finally, previous studies testing the JH + SHE combination revealed unexpected increases in soldier caste differentiation relative to treatments with JH alone, whereas treatments with SHE alone had no such impacts [7,12,18]. In the current study, we conducted “follow-up” bioassays and qRT...
Animals were habituated to the testing room 1 h prior to the test. The difference between T1 and T2 reflected stress-induced hyperthermia. 2.11. Intestinal motility assay Gastrointestinal motility was assessed as previously described (Golubeva et al., 2017). Briefly, mice were single-housed at ...
22 Following 3 days of training, mice underwent 3 days of testing without food in the nose-pokes, during which the number of active and inactive nose pokes was measured over a 30-min test period each day. Microdialysis experiments All mice were acclimated to microdialysis chambers for 4 h ...