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How long does Viome testing take? Microbiome testing with Viome takes approximately five weeks: about one week to receive your kit, collect your sample, and ship it back, and about four weeks to process the sample completely. Blood sample testing takes a bit longer than stool testing. These ...
children were instructed to collect fecal samples using an at-home collection kit that has been tested in our project with > 80% compliance [31]. Parents received instructions to assist their child to collect fecal samples. The provided spoon was used to transfer ...
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(three-fold, repeated ten times) stratified for disease status where the training and testing data were separate in each iteration. The prevalidated predicted values in the testing sets based on the optimal cross-validated models trained on species-level CLR abundances were used as the gut ...
The urine specimens were centrifuged at 2000 × gfor 30 min on the same day of collection and the urine supernatant was stored at −80 °C in 1 mL aliquots. Cell-free DNA was extracted from the urine using the Qiagen Circulating Nucleic Acid Kit (Qiagen). Extracted cell-free...
Prior to testing, mice were fasted overnight (∼16 h). Using a triple-crossover study design, all mice were randomized to receive an oral gavage of water (vehicle), 50 mg/kgmetformin, or 150 mg/kgmetformin. Treatments were switched for subsequent tests, occurring 2 weeks apart for suffi...
DNA extraction, 16S rRNA gene amplicon sequencing, and inference of bacterial ASVs Bacterial DNA from both FACS-sorted cells (6 h) and fecal incubations (0 and 6 h) were extracted using QIAamp DNA Mini Kit (Qiagen, Germany) following the protocol for bacteria according to the ...
As MaAsLin performs paralleled multiple analyses, q < 0.05 (false discovery rate (FDR) multiple testing corrected) was used as significance threshold. All MaAsLin models were adjusted for technical covariates and other confounders as described in the sections below. Technical covariates in the ...
vegan R package) computed on Jaccard distance matrix (p < 0.05). Comparisons of taxa or gene abundance between groups were carried out using pairwise Wilcoxon tests. Statistical significance of pangene prevalence was verified through Fisher’s test with multiple-hypothesis testing corrections via...