Library preparation and sequencing was conducted at the Integrated Microbiome Resource, Dalhousie University, as described in Comeau et al.43. The V4–V5 region of the 16S rRNA gene was amplified by PCR using the 515F–926R primers44,45. All samples were amplified and sequenced in one batch...
Similarly, Integrase genes (int) are important for gut microbiome homeostasis because they allow viruses to transfer genes to bacteria which may enhance bacterial metabolic functions, eliminate competing organisms, or increase virulence34. For example, some viruses enhance bacterial metabolic functions by ...
Taxonomic and functional analysis at genus and species level revealed significant changes in the gut microbiota composition, carbohydrate metabolism specifically, CAZy genes. Diversity of gut microbes and CAZy associated functional genes form the basis for maintaining homeostasis of the gut microbiome and h...
Neu J, Lorca G, Kingma SDK, Triplett EW: The Intestinal Microbiome: Relationship to Type 1. Diabetes. 2010, 39: 563-571. CAS Google Scholar Tiittanen M, Huupponen JT, Knip M, Vaarala O: Insulin treatment in patients with type 1 diabetes induces upregulation of regulatory T-cell mark...
Obesity and related comorbidities are major health concerns among many US immigrant populations. Emerging evidence suggests a potential involvement of the gut microbiome. Here, we evaluated gut microbiome features and their associations with immigration,
Furthermore, longitudinal observational studies provide additional evidence that recolonization of the gut may potentially play a critical role in microbiome recovery from antibiotic-exposure. However, more studies are needed which are designed to test the specific hypotheses suggested by these few studies...
While our research provides new and important insights, there's still a lot to learn. Many regions, including parts of South America and Africa, are underrepresented in microbiome studies. This limits our understanding of howgut bacteriavary across different populations. ...
Gut microbiome 16S Oxford Nanopore Technologies Protozoan microorganisms Diarrhoea Italy 1. Introduction The human gut microbiome harbours a large number of microorganisms such as viruses, bacteria, fungi, and protozoa (single-celled eukaryotes). They are involved widely and differentially within various ...
Here we present a large comparison of gut microbiome profiles in children from the Democratic Republic of the Congo, using shotgun metagenomic sequencing, with study populations ranging from the urbanized capital of Kinshasa to the extremely rural settings of south-western DRC, including children affec...
Gut bacteria from the Enterobacteriaceae family are a major cause of opportunistic infections worldwide. Given their prevalence among healthy human gut microbiomes, interspecies interactions may play a role in modulating infection resistance. Here we unc