sgRNA stands for single guide RNA, as opposed to the conventional, native S. pyogenes CRISPR-Cas9 system that uses a two-part guide in which CRISPR RNA (crRNA) is hybridized with a tracrRNA. An sgRNA combines both RNAs into a single, long RNA. sgRNAs are typically 100 nt in length and...
百度试题 结果1 题目CRISPR/Cas9需要guideRNA,它起什么作用?( ) A. 催化Cas9酶切靶向DNA B. 引导Cas9酶识别目的DNA片段 C. 稳定Cas9蛋白构象 D. 提供PAM(protospacer-adjacent motif) 相关知识点: 试题来源: 解析 B 反馈 收藏
The CRISPR/Cas9 system has been rapidly adopted for genome editing. However, one major issue with this system is the lack of robust bioinformatics tools for design of single guide RNA (sgRNA), which determines the efficacy and specificity of genome editi
The CRISPR/Cas9 system has been rapidly adopted for genome editing. However, one major issue with this system is the lack of robust bioinformatics tools for design of single guide RNA (sgRNA), which determines the efficacy and specificity of genome editing. To address this pressing need, we an...
CRISPR-Cas (聚类,定期 Interspaced,短 Palindromic 联系 RepeatsCRISPR (Cas )) RNA 指导了 endonuclease 作为编辑技术的最有效,广泛地使用的染色体出现了,它成为了最令人激动,很快进展的研究地.有效染色体由 CRISPR-Cas9 系统编辑在许多种类被表明了,并且几个实验室为系统的基因分析作为一个屏蔽工具建立了 CRISPR-...
A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome edit- ing. Sci Rep. 2015;5:14926.Yin K, Han T, Liu G, Chen T, Wang Y, Yu A Y, Liu Y. A gemi- nivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing. Sci Rep, ...
CRISPR-Cas9基因组编辑技术是一种强大的工具,能够实现目标基因的调控。虽然这一技术能实现针对目标基因的高效编辑,但是同时也存在无法忽视的脱靶效应。在本文中,作者发展了一种基于RNA化学修饰以及光激活生物正交反应的针对sgRNA的修饰调控方法...
MolecularPlant · Volume7 · Number5· Pages923—926· May2014 LE1_rERTOTHEEDITOR Genome--WidePredictionofHighlySpecific GuideRNASpacersforCRISPR-Cas9·-Mediated GenomeEditinginModelPlantsandMajor Crops DearEditor, RNA—guidedgenomeediting(RGE)usingtheStreptococcus pyogenesCRISPR-Cas9system(Jineketa1.,201...
09 October 2015 A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing Kangquan Yin1,2, Ting Han1, Guang Liu1, Tianyuan Chen1, Ying Wang1, Alice Yunzi L. Yu1,† & Yule Liu1 CRISPR/Cas has emerged as potent genome editing te...
声明: 本网站大部分资源来源于用户创建编辑，上传，机构合作，自有兼职答题团队，如有侵犯了你的权益，请发送邮箱到feedback@deepthink.net.cn 本网站将在三个工作日内移除相关内容,刷刷题对内容所造成的任何后果不承担法律上的任何义务或责任