Zhu LJ. Overview of guide RNA design tools for CRISPR-Cas9 genome editing technology. Front Biol. 2015;10(4):289-96.Zhu LJ. Overview of guide RNA design tools for CRISPR-Cas9 genome editing technology. Front Biol (Beijing). 2015;10:289-96. https://doi.org/10. 1007/s11515-015-1366-...
美国科学家团队开发出适应于CRISPR/Cas13系统的guide RNA设计体系,研究成果于2020年3月16日在《自然·生物技术》上在线发表。研究表明,gRNA存在保证其靶向活性的核心区域,其中心位于相对于gRNA 5’端第18个核苷酸,并发现当错配位于靶标位点...
sgRNA stands for single guide RNA, as opposed to the conventional, native S. pyogenes CRISPR-Cas9 system that uses a two-part guide in which CRISPR RNA (crRNA) is hybridized with a tracrRNA. An sgRNA combines both RNAs into a single, long RNA. sgRNAs are typically 100 nt in length and...
sgRNA stands for single guide RNA, as opposed to the conventional, nativeS. pyogenesCRISPR-Cas9 system that uses a two-part guide in which CRISPR RNA (crRNA) is hybridized with a tracrRNA. An sgRNA combines both RNAs into a single, long RNA. sgRNAs are typically 100 nt in length and can...
CRISPR/Cas系统是细菌和古细菌在不断进化的过程中产生的适应性免疫防御机制,它应用CRISPR RNA(crRNA)以碱基互补的形式引导相应的Cas蛋白识别入侵的外源基因组,并对其DNA进行剪切,用以保护自身的基因组免受外源核酸如噬菌体、病毒等的干扰和破坏。经过人为改造后,可在真核细胞中实现高度灵活且特异的基因组编辑,通过设计...
增强基因敲除信心Dharmacon Edit-R CRISPR系列guide RNA是经验证的算法设计的,可实现高特异性的功能基因敲除,因此您可以对敲除结果充满信心。 适应多种工作流程的灵活性DNA-free体系:合成sgRNA或crRNA: tracrRN…
在HeLa细胞中进行了全基因组CRISPR-Cas9筛选，GeCKO v2向导RNA ( gRNA )文库并进行3轮TcdB4选择 2. ...
所有Edit-R预先设计的指导RNA(合成的crRNA和慢病毒sgRNA)都设计有经过验证的算法,以提高功能性敲除的可能性,而不仅仅是双链断裂(DSB)。 单独的位置不是CRISPR靶位点功能的指示 沿着VCP基因的编码序列的长度评估crRNA的功能性表明没有特定的模式,因为它涉及外显子位置,增强了设计算法表征功能特征的重要性。重组U2OS ...
The CRISPR Guide RNA design tool allows you to visualize, optimize, and annotate multiple gRNA sequences at a time. Get on and off-target scores in seconds to compare and optimize for higher activity and lower off-target effects. Save, organize and attach guides to relevant sequences and ass...
Download the CRISPR Basics Handbook How does our gRNA design tool work? The IDT Custom Alt-R™ CRISPR-Cas9 guide RNA design tool can be used to help you design gRNA sequences for use with Cas9 with high on-target and low off-target activity in human, mouse, rat, zebrafish, and C. ...