We demonstrate application of ECCITE-seq to multimodal CRISPR screens with robust direct single-guide RNA capture and to clonotype-aware multimodal phenotyping of cancer samples. less Expand Protocol Nature Protocols (2019) Integrative analysis of pooled CRISPR genetic screens using MAGeCKFlute ...
crisprDesignis the core package of thecrisprVerseecosystem, and plays the role of a one-stop shop for designing and annotating CRISPR guide RNA (gRNA) sequences. This includes the characterization of on-targets and off-targets using different aligners, on- and off-target scoring, gene context ...
Benchling's CRISPR tool eliminates tedious work by batching gRNA design and executing it up to 100x faster than leading competitor Save and export guide sequences Save your guides as oligos on Benchling, organize them using tags and folders, and clone them into CRISPR plasmids Alternatively, export...
为了能够实现更简便的特定基因序列编辑,研究者将CRISPR/Cas9系统优化为Cas9内切酶和单导向RNA(single-guide RNA, sgRNA)。sgRNA是tracrRNA和crRNA复合分子[18],其中crRNA包含PAM序列和20 nt左右碱基对的靶向序列[9]。sgRNA通过PAM序列...
gRNA, or guide RNA, is part of the CRISPR-Cas9 system. The gRNA molecule has two components: a target-specific CRISPR RNA (crRNA) and an auxiliary trans-activating crRNA (tracrRNA). The gRNA guides the Cas9 protein to a specific genomic locus via base pairing between the 20-mer crRNA seq...
A Computational Workflow for Designing Libraries of sgRNAs for CRISPR-Mediated Base Editing, and much more crisprbase-editinggenome-wide-targeted-mutagenesisguide-rna-library UpdatedMay 26, 2024 Python A software tool for personalized and allele-specific CRISPR editing. ...
There are many excellent tools for designing CRISPR-Cas guide RNAs (gRNAs) for model organisms with standard Cas enzymes. GuideMaker is intended as a fast and easy-to-use design tool for challenging projects with (i) non-standard Cas enzymes, (ii) non-model organisms, or (iii) projects ...
guide sequenceCRISPR/Cas technology of genome editing is a powerful tool for making targeted changes in the DNA of various organisms, including plants. The choice of the precise nucleotide sequence (protospacer) in the gene to be edited is important in the design of guide RNA, which can be ...
immunity, ShoINT and random fragmentation NGS experiments. E.E.C. helped with cloning and transposition experiments. C.A. assisted with computational analyses of NGS data and the guide RNA design algorithm. P.L.H.V., S.H.S. and all other authors discussed the data and wrote the ...
Protect your RNA workflows with reliable and efficient essential RNA enzymes. T7 Polymerase This robust RNA polymerase, derived from the T7 bacteriophage, transcribes RNA from DNA sequences under the control of the T7 promoter. Learn More ...