Protect your RNA workflows with reliable and efficient essential RNA enzymes. T7 Polymerase This robust RNA polymerase, derived from the T7 bacteriophage, transcribes RNA from DNA sequences under the control of the T7 promoter. Learn More ...
guide sequenceCRISPR/Cas technology of genome editing is a powerful tool for making targeted changes in the DNA of various organisms, including plants. The choice of the precise nucleotide sequence (protospacer) in the gene to be edited is important in the design of guide RNA, which can be ...
The CRISPR Guide RNA design tool allows you to visualize, optimize, and annotate multiple gRNA sequences at a time. Get on and off-target scores in seconds to compare and optimize for higher activity and lower off-target effects. Save, organize and attach guides to relevant sequences and ass...
CRISPR/Cas9系统实现基因敲除绝大部分外显子,定制服务 CRISPR/Cas9 系统中,Cas9 蛋白是一种核酸酶,它能够在向导 RNA(gRNA)的引导下对特定的 DNA 序列进行切割。gRNA 包含一段与目标 DNA 互补的序列,通过碱基互补配对原则,将 Cas9 蛋白精准地带到目标基因的外显子区域。 当Cas9 蛋白切割 DNA 双链后,会产生双链...
It utilizes a DNA endonuclease, Cas9, and a guide RNA (gRNA) to make specific cleavages in the DNA at desired locations. This system has been rapidly applied to edit genes in various species, including humans. CRISPR-Cas9 is commonly used for gene knockouts, which involve introducing small ...
CRISPR/Cas9 系统主要由 Cas9 核酸酶和向导 RNA(gRNA)组成。gRNA 能够与目标 DNA 序列互补配对,引导 Cas9 蛋白定位到特定的 DNA 区域。当 Cas9 结合到目标外显子的特定序列后,发挥其核酸内切酶活性,切割 DNA 双链,产生双链断裂(DSB)。细胞会通过非同源末端连接(NHEJ)来修复 DSB,这个过程容易产生插入或缺失(inde...
immunity, ShoINT and random fragmentation NGS experiments. E.E.C. helped with cloning and transposition experiments. C.A. assisted with computational analyses of NGS data and the guide RNA design algorithm. P.L.H.V., S.H.S. and all other authors discussed the data and wrote the ...
CRISPR/Cas9基因编辑系统主要由两部分组成:相当于“扳手”作用的Cas9蛋白与相当于“螺纹钉”作用的CRISPR向导RNA(guide RNA,gRNA)。“螺纹钉”负责对靶位点进行定位,并招募和激活Cas9蛋白;Cas9蛋白则负责切割DNA(图2)。 图2. "分子改造器" CRISPR/Cas9
与传统的RNAi(RNA interference)相比,Cas9最大的优势在于更低的脱靶率。基于Cas9诸多优势,全球各个实验室利用Cas9技术在多种细胞系及各种模式动物上进行实验,成果显著。然而,单纯的设计一条或多条sgRNA(single guide RNA)进行基因敲低,这样的实验方法通量过低,因此,借鉴shRNA Screen技术,许多团队开发出版本各异的Cas9...
Methods Cell culture, electroporation, transfection Cell line-specific culture and manipulation protocols described in Supplementary Data 1. All parental cell lines were sourced from the Genentech cell bank (gCell) where they were maintained under mycoplasma-free conditions and authenticated by STR profili...