Biochemical Identification of an RLD-Containing GEF, GLO-4, as an RPM-1 Binding Protein To identify signaling pathways regulated by RPM-1, we performed a biochemical assay to search for RPM-1 interacting proteins (Figure 1B). We used a transgenic line (juIs58) expressing RPM-1 fused with...
Cell Invasion Assay A BD BioCoatTM MatrigelTM Invasion Chamber (BD Biosciences) was used for the invasion assay. Cells (2.5 × 104) were seeded per insert in triplicate in 500 μl of serum-free McCoy's 5A medium. Inserts were placed in wells containing 500 μl of medium with 5% FBS ...
Alternatively, gene expression profiling according to the prediction analysis of microarray (PAM) 50 assay [3] can subdivide BC into intrinsic subtypes Cancers 2019, 11, 101; doi:10.3390/cancers11010101 www.mdpi.com/journal/cancers Cancers 2019, 11, 101 2 of 17 characterized by differential ...