产品名称:GTPase-Glo? Assay .0订购此产品 供应商:Promega 规格:10,000 Assays 目录价:18735 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址:点击查看详细 打印此页关闭此页 上一个:GTPase-Glo? Assay[V7681/1,000 Assays] ...
By measuring the amount of GTP that remains after the hydrolysis reaction, the GTPase-Glo assay enables evaluation of the activity of GTPases, GAP proteins, and GEF. Using the GTPase-Glo Reagent, unhydrolized GTP is transformed into ATP. Subsequently, luciferase uses ATP as a substrate for ...
4181Accesses 10Altmetric Metrics Abstract The establishment of cell polarity is a prerequisite for many developmental processes. However, how it is achieved during tip growth in plants remains elusive. Here, we show that the RHO OF PLANTs (ROPs), ROP GUANINE NUCLEOTIDE EXCHANGE FACTORs (RopGEFs),...
Nonalcoholic fatty liver disease (NAFLD) is a condition in which excess fat is stored in the liver in people who drink little or no alcohol. NAFLD has become the most common cause of liver disease worldwide with an estimated global prevalence of 25.24%1. Isolated hepatosteatosis is the most ...
Cytokine-dependent growth was determined by quantification of cellular ATP using the Cell Titer Glo Assay (Promega, Madison, WI, USA). Cells were washed with RPMI and starved for 3 hours in the presence of 1 mg/ml BSA. 3.75 × 104 cells/ml were seeded in a 96 well plate with the corr...
Four yeast proteins, Gcs1, Glo3, Age2, and Age1, have been shown to exert ArfGAP activity in vitro (8, 9, 10, 11), with the Age2 + Gcs1 ArfGAP pair providing essential overlapping function for post-Golgi transport (10). Vesicular transport is also mediated by membrane lipid ...
Forty-eight hours after transfection, cells were analyzed for luciferase activity using the Dual-Glo Luciferase Assay System (Promega). QGP1 cells were transfected with pmirGLO Dual-Luciferase 3′ UTR vector using Lipofectamine 3000 Transfection Agent, following the manufacturer's instructions (Invitrogen...
Protein concentration of supernatant was estimated using the bicinchoninic acid (BCA) protein assay (Pierce Biotechnology, Rockford, Illinois, USA), according to the manufacturer's instructions. Equal amounts of protein (30 μg) were separated by use of SDS-polyacrylamide gel electrophoresis (SDS-...
Several multi-domain GEFs which activate Rap1 and cell junction formation have been identified and characterized, including C3G, Epacs, PDZ-GEFs, RapGAPs and DOCK4 (Gloerich and Bos, 2011). Likewise, a number of downstream effector proteins work in concert with Rap1 to regulate cell adhesive...
Biochemical Identification of an RLD-Containing GEF, GLO-4, as an RPM-1 Binding Protein To identify signaling pathways regulated by RPM-1, we performed a biochemical assay to search for RPM-1 interacting proteins (Figure 1B). We used a transgenic line (juIs58) expressing RPM-1 fused with...