Yeast GTP-binding protein (YPT1 protein) has been reported to function in the early stages of the secretory pathway. Particularly, YPT1 protein is observed to regulate both the endoplasmic reticulum-to-Golgi transport and the autophagy. Therefore, the YPT1 protein overexpressed in yeast vacuoles...
Fairley-Grenot K, Assmann SM: Evidence for G-protein regulation of inward K+channnel current in guard cells of fava bean. Plant Cell 3: 1037–1044 (1991). ArticlePubMedGoogle Scholar Fawell E, Hook S, Sweet D, Armstrong J: Novel YPT1-related genes fromSchizosaccharomyces pombe. Nucl Acid...
We report the cloning of a low-molecular-weight GTP-binding protein that appears to be the first member of a new class of G proteins. This G protein was cloned from the HT4 neural cell line and has the closest homology to the rab, sec4, and ypt1 members of the low-molecular-weight...
A set of 11 clones encoding putative GTP binding proteins highly homologous to the yeast YPT1 SEC4 gene products have been isolated from an MDCK cell cDNA library. We localized three of the corresponding proteins in mammalian cells by using affinity-purified antibodies in immunofluorescence and imm...
Article: The small GTP-binding protein Rho regulates a phosphatidylinositol 4-phosphate 5-kinase in mammalian cells
Except for the N-terminal DCB domain which is also found in the yeast protein Ysl2p [28], all of them are unique to these two ArfGEFs subfamilies within the detection limits of the BLAST search. The HUS domain features a remarkably conserved N(Y/F)DC(D/N) motif, which we call the...
The SAR coding region with a green fluorescent protein (GFP) tag at the 3′ end or GFP alone was subcloned into the murine stem cell virus (MSCV) retroviral expression vector (Clontech). The retroviruses were packaged into PT67 packaging cells. The viral titers were determined and high-titer...
A yeast GTP-binding protein, the YPT1 gene product, has been found to function early in the secretion pathway. The ypt1-1mutation causes a phenotype reminiscent of early secretion-defective mutants, including accumulation of membranes and vesicles as well as a partial defect in secretion and ...
Interactions of three domains distinguish- ing the Ras-related GTP-binding proteins Ypt1 and Sec4. Nature. 362:560-563.Brennwald, P. and Novick, P. (1993) Interactions of three domains distinguishing the Ras-related GTP-binding proteins Ypt1 and Sec4. Nature 362, 560-563...
Interactions of three domains distinguishing the Ras-related GTP-binding proteins Ypt1 and Sec4. Nature. 362:560- 563.Brennwald, P., and Novick, P. (1993). Interactions of three domains distinguishing the Ras-related GTP-binding proteins Ypt1 and Sec4. Nature 362, 560-563....